96-WELL FORMAT-BASED MICROFLUIDIC PLATFORM FOR HIGH-THROUGHPUT DRUG SCREENING

Abstract

In this study, a simple and robust microfluidic platform is presented, which can interconnect and culture multiple microtissues (MTs) as 96-well format-based platform. Human colon cancer (HCT116) MTs were cultured over 4 days and exposed to various concentrations of endoxifen. Drug resistance was observed when HCT116 MTs were cultured under perfusion conditions. It addresses the feasibility of our microfluidic platform for more complex and comprehensive pharmacokinetic study in an in vivo-like in vitro physiological environment. © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.