Mag-centrifugal microfluidic separation for rapid purification of dorsal root ganglion neurons

Abstract

Dorsal root ganglion (DRG) neurons have been widely used as a good model for sensory neurons of the peripheral nervous system in neurology. However, purification process is essential to only acquire the sensory neurons since the DRG organ is composed of several types of cells including fibroblast, Schwann and epithelial cells. In this study, we introduce a novel hybrid isolation method combining centrifugal and immunomagnetic separations to selectively remove the Schwann cells, fibroblasts and epithelial cells with fast manner. As a result, we obtained high purity and high yield of primary sensory neurons and achieved a reliable and rapid purification method through an automated process. Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved.