Cell block preparation from pleural fluid by an ultrasound-assisted filtration

Abstract

This paper reports a novel ultrasound-assisted filtration technique for cell block (CB) preparation from pleural fluid. CB (> 5×106 cells) from a large-volume (>10 mL) specimen was obtained on the Parylene-C filtration membrane (1.29×106 micropores) with a high recovery rate (>90%), a high cell viability (>96%) and an effective depletion of red blood cells (RBCs). The prepared CB of A549s (cultured lung cancer cells) from PBS and target cells from clinical pleural fluid can be well embedded with paraffin. Successful hematoxylin-eosin (HE), immunocytochemistry (ICC) and immunofluorescence (IF) staining of the CBs sections were demonstrated, showing the promise of application in clinical practices. Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved.