Extracellular vesicles expressing cytokines and antibodies, method for producing same, and use thereof

Abstract

The present invention relates to extracellular vesicles expressing cytokines and antibodies, methods of making said extracellular vesicles, and uses thereof. Provided is a method for producing an immune cell expressing both a cytokine and an antibody on the surface of a cell membrane by transforming the immune cell with a first vector containing a gene encoding the cytokine and a second vector containing a gene encoding the antibody, and a method for producing an extracellular vesicle that expresses both a cytokine and an antibody on the surface from the immune cell. By combining a first vector and a second vector so that a specific cytokine or a specific antibody can be expressed according to a target cell, the present invention provides an extracellular vesicle that can induce a desired cytokine response in a cell that can be targeted by a specific antibody, and that is safe to the human body, meanwhile, an excellent anti-cancer effect is shown. The present invention provides a novel cancer treatment means that can be used to deliver a drug or a physiologically active substance to a specific cancer cell by encapsulating the drug or the physiologically active substance having a therapeutic effect on cancer within extracellular vesicles.