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The present invention provides a cell culture apparatus. A cell culture apparatus according to one embodiment of the present invention is a cell culture apparatus for culturing a cell to form the cell into a three-dimensional spheroid. The cell culture apparatus may comprise: a plate; and a well which is arranged, in plural, on one side of the plate at predetermined intervals, has cells cultured inside, and has a micropattern disposed on the inner surface such that the inner surface and the cells are spaced apart from each other.
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