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The ELAVL1 Regulates mRNA Export and Nucleocytoplasmic Trafficking in Cellular Senescence

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dc.contributor.advisor 이영삼 -
dc.contributor.author Yeeun Cho -
dc.date.accessioned 2026-01-23T10:54:58Z -
dc.date.available 2026-01-23T10:54:58Z -
dc.date.issued 2026 -
dc.identifier.uri https://scholar.dgist.ac.kr/handle/20.500.11750/59658 -
dc.identifier.uri http://dgist.dcollection.net/common/orgView/200000947584 -
dc.description Cellular Senescence, mRNA Export, Nucleocytoplasmic Trafficking, ELAVL1 -
dc.description.tableofcontents Ⅰ. Introduction 1
1.1 Aging and Cellular Senescence 1
1.2 Molecular Hallmarks of Cellular Senescence 2
1.3 Regulation of Gene Expression during Aging 3
1.4 Post-transcriptional Regulation and RNA-binding Proteins in Aging 3
1.5 Nucleocytoplasmic Trafficking and Its Dysregulation During Aging 5
1.6 ELAVL1 and Its Associated Complexes 6
1.7 Overview of This Study 9

Ⅱ. Experimental Method & Materials 10
2.1 Cell culture 10
2.2 Plasmid construction and transfection 10
2.2.1 Lentivirus-mediated knockdown and overexpression 10
2.2.2 Establishment of stable cell lines 11
2.3 Western blot analysis 11
2.4 Cell proliferation assay using EdU 12
2.5 Reactive oxygen species (ROS) assay 12
2.6 SA-β-Gal staining 13
2.6.1 Detection of SA-β-Gal activity 13
2.6.2 Quantification of SA-β-Gal-positive cells using FDG staining 13
2.7 Subcellular fractionation 14
2.8 RNA extraction and RT-qPCR 14

Ⅲ. Results 15
3.1 Identification of ELAVL1 as a potential regulator of cellular senescence 15
3.2 Downregulation of ELAVL1, PP32, and APRIL in senescent HDFs 19
3.3 Knockdown of ELAVL1, PP32, and APRIL expression alters senescence-associated 22
3.4 Knockdown of ELAVL1 leads to nuclear accumulation of WSB1 mRNA 29

Ⅳ. Discussion 35

Ⅴ. References 40

요 약 문 47		 -
dc.format.extent 47 -
dc.language eng -
dc.publisher DGIST -
dc.title The ELAVL1 Regulates mRNA Export and Nucleocytoplasmic Trafficking in Cellular Senescence -
dc.title.alternative ELAVL1 에 의한 mRNA 수출 및 핵-세포질 이동 조절과 세포 노화에서의 역할 -
dc.type Thesis -
dc.identifier.doi 10.22677/THESIS.200000947584 -
dc.description.degree Master -
dc.contributor.department Department of New Biology -
dc.contributor.coadvisor Yun-Il Lee -
dc.date.awarded 2026-02-01 -
dc.publisher.location Daegu -
dc.description.database dCollection -
dc.citation XT.NM 조64 202602 -
dc.date.accepted 2026-01-19 -
dc.contributor.alternativeDepartment 뉴바이올로지학과 -
dc.subject.keyword Cellular Senescence, mRNA Export, Nucleocytoplasmic Trafficking, ELAVL1 -
dc.contributor.affiliatedAuthor Yeeun Cho -
dc.contributor.affiliatedAuthor Young-Sam Lee -
dc.contributor.affiliatedAuthor Yun-Il Lee -
dc.contributor.alternativeName 조예은 -
dc.contributor.alternativeName Young-Sam Lee -
dc.contributor.alternativeName 이윤일 -
dc.rights.embargoReleaseDate 2027-02-28 -
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