Title

The ELAVL1 Regulates mRNA Export and Nucleocytoplasmic Trafficking in Cellular Senescence

Alternative Title

ELAVL1 에 의한 mRNA 수출 및 핵-세포질 이동 조절과 세포 노화에서의 역할

DGIST Authors

Yeeun Cho ; Young-Sam Lee ; Yun-Il Lee

Advisor

이영삼

Co-Advisor(s)

Yun-Il Lee

Issued Date

2026

Awarded Date

2026-02-01

Type

Thesis

Description

Cellular Senescence, mRNA Export, Nucleocytoplasmic Trafficking, ELAVL1

Table Of Contents

Ⅰ. Introduction 1
1.1 Aging and Cellular Senescence 1
1.2 Molecular Hallmarks of Cellular Senescence 2
1.3 Regulation of Gene Expression during Aging 3
1.4 Post-transcriptional Regulation and RNA-binding Proteins in Aging 3
1.5 Nucleocytoplasmic Trafficking and Its Dysregulation During Aging 5
1.6 ELAVL1 and Its Associated Complexes 6
1.7 Overview of This Study 9

Ⅱ. Experimental Method & Materials 10
2.1 Cell culture 10
2.2 Plasmid construction and transfection 10
2.2.1 Lentivirus-mediated knockdown and overexpression 10
2.2.2 Establishment of stable cell lines 11
2.3 Western blot analysis 11
2.4 Cell proliferation assay using EdU 12
2.5 Reactive oxygen species (ROS) assay 12
2.6 SA-β-Gal staining 13
2.6.1 Detection of SA-β-Gal activity 13
2.6.2 Quantification of SA-β-Gal-positive cells using FDG staining 13
2.7 Subcellular fractionation 14
2.8 RNA extraction and RT-qPCR 14

Ⅲ. Results 15
3.1 Identification of ELAVL1 as a potential regulator of cellular senescence 15
3.2 Downregulation of ELAVL1, PP32, and APRIL in senescent HDFs 19
3.3 Knockdown of ELAVL1, PP32, and APRIL expression alters senescence-associated 22
3.4 Knockdown of ELAVL1 leads to nuclear accumulation of WSB1 mRNA 29

Ⅳ. Discussion 35

Ⅴ. References 40

요 약 문 47

URI

https://scholar.dgist.ac.kr/handle/20.500.11750/59658
http://dgist.dcollection.net/common/orgView/200000947584

DOI

10.22677/THESIS.200000947584

Degree

Master

Department

Department of New Biology

Publisher

DGIST

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