Title

Role of KCNK1 in Alveolar Type 2 Cell Stemness: Insights from Organoid and In Vivo Bleomycin-Induced Lung Injury

Alternative Title

폐포 제2형 세포의 줄기성 조절에서 KCNK1의 역할: 오가노이드 및 블레오마이신 유도 폐 손상 모델을 통한 분석

DGIST Authors

Yeonhyo Choi ; Youngtae Jeong ; Jaemin Lee

Advisor

정영태

Co-Advisor(s)

Jaemin Lee

Issued Date

2026

Awarded Date

2026-02-01

Type

Thesis

Description

KCNK1, Alveolar type 2, Stem cell, Organoid

Table Of Contents

Ⅰ. Introduction 1
1.1 Stem cell 1
1.2 Physiological Background of Alveolar Regeneration and Lung Injury 2
1.3 Heterogeneity of AT2 Stem Cells and the Alveolar Stem Cell Niche 4
1.4 Potassium channel and KCNK1 6
1.5 Organoid model 8
1.6 Research Objective and Overview 10
Ⅱ. Materials and Methods 14
2.1 Mouse alveolar cell isolation 14
2.2 Genotyping· 15
2.3 Quantitative Real-Time PCR (qPCR) 17
2.4 Alveolar organoid culture 18
2.5 Tamoxifen administration and Bleomycin-induced lung injury 21
2.6 Tissue processing, histology, and immunofluorescence 22
2.7 Single-cell RNA-seq analysis 24
2.8 Statical analysis 25
Ⅲ. Result 26
3.1 Single-cell transcriptomic analysis of KCNK1 26
3.2 Kcnk1 Deficiency Impairs Alveolar Organoid Formation 27
3.3 Acute Lung Injury Day 4 after Bleomycin Administration 28
3.4 Fibrotic Remodeling at Two Weeks after Bleomycin-Induced Injury 29
3.5 Lineage Tracing of AT2-to-AT1 Transition after Bleomycin-Induced Injury30
Ⅳ. Discussion 38
Reference 42
요약문 47

URI

https://scholar.dgist.ac.kr/handle/20.500.11750/59659
http://dgist.dcollection.net/common/orgView/200000946013

DOI

10.22677/THESIS.200000946013

Degree

Master

Department

Department of New Biology

Publisher

DGIST

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