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  Department of Brain Sciences Theses Master

Human iPSC-Derived Assembloid of Sensory Pathway: A Platform for Investigating E/I Imbalance in Autism Spectrum Disorders

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dc.contributor.advisor 이성배 -
dc.contributor.author Juwon Lee -
dc.date.accessioned 2026-01-23T11:01:13Z -
dc.date.available 2026-01-23T11:01:13Z -
dc.date.issued 2025 -
dc.identifier.uri https://scholar.dgist.ac.kr/handle/20.500.11750/59811 -
dc.identifier.uri http://dgist.dcollection.net/common/orgView/200000889586 -
dc.description induced pluripotent stem cells (iPSCs), assembloid, thalamocortical circuit, microglia, autism spectrum disorder (ASD) -
dc.description.tableofcontents Ⅰ. Introduction 1
1.1 Brain Organoids as a Human-Based Model 1
1.2 Assembloid for Modeling Complex Brain Circuits 2
1.3 Autism Spectrum Disorder 3
1.3.1 Sensory abnormalities and ASD 4
1.3.2 ASD-related genes 5
1.4 Challenges in Studying ASD in Humans 7
IⅠ. Materials and Methods 11
2.1 Cell culture 11
2.1.1 Human induced pluripotent stem cells (hiPSCs) 11
2.1.2 Generation of ventral thalamic organoid (vThO), ganglionic eminence organoid (GeO), and cerebral cortex organoid (CxO) from hiPSCs 11
2.1.3 HPC Differentiation from iPSCs 13
2.1.4 Microglia Differentiation from HPC 14
2.1.5 CRISPR/Cas9-mediated gene editing of CNTNAP2 and SHANK3 15
2.2 Generation of assembloid 16
2.3 Cryosection and immunohistochemistry 16
2.4 Real-time qPCR 17
ⅠII. Results 18
3.1 Previous Modeling Strategies and Opportunities for Extension 18
3.2 Designing the structure of CS assembloid and modifying protocols 23
3.3 Optimization of culture conditions for ventral thalamic organoids 27
3.4 Establishing a CS assembloid by fusing ventral thalamic, ganglionic eminence, and cortical organoids 32
3.5 iPSC-derived microglia like cells in Cerebral cortex organoid 37
3.6 CRISPR/Cas9-based targeting for CNTNAP2 and SHANK3 in iPSCs 42
IV. Disccusion 46
V. References 49
Abstract in Korean 53		 -
dc.format.extent 53 -
dc.language eng -
dc.publisher DGIST -
dc.title Human iPSC-Derived Assembloid of Sensory Pathway: A Platform for Investigating E/I Imbalance in Autism Spectrum Disorders -
dc.title.alternative 중추 신경계 감각 경로 모델링: 자폐스펙트럼장애의 흥분성/억제성 신경망 불균형 연구를 위한 인간 유도만능줄기세포 유래 어셈블로이드 -
dc.type Thesis -
dc.identifier.doi 10.22677/THESIS.200000889586 -
dc.description.degree Master -
dc.contributor.department Department of Brain Sciences -
dc.contributor.coadvisor Jinsoo Seo -
dc.date.awarded 2025-08-01 -
dc.publisher.location Daegu -
dc.description.database dCollection -
dc.citation XT.BM 이76 202508 -
dc.date.accepted 2025-07-21 -
dc.contributor.alternativeDepartment 뇌과학과 -
dc.subject.keyword induced pluripotent stem cells (iPSCs), assembloid, thalamocortical circuit, microglia, autism spectrum disorder (ASD) -
dc.contributor.affiliatedAuthor Juwon Lee -
dc.contributor.affiliatedAuthor Sung Bae Lee -
dc.contributor.affiliatedAuthor Jinsoo Seo -
dc.contributor.alternativeName 이주원 -
dc.contributor.alternativeName Sung Bae Lee -
dc.contributor.alternativeName 서진수 -
dc.rights.embargoReleaseDate 2030-08-31 -
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