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  Department of Brain Sciences Theses Master

Investigating the excitatory-inhibitory imbalance in Alzheimer's disease using cerebral cortex and ganglionic eminence fusion organoids

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dc.contributor.advisor 이성배 -
dc.contributor.author Juran Choe -
dc.date.accessioned 2026-01-23T11:01:16Z -
dc.date.available 2026-01-23T11:01:16Z -
dc.date.issued 2025 -
dc.identifier.uri https://scholar.dgist.ac.kr/handle/20.500.11750/59812 -
dc.identifier.uri http://dgist.dcollection.net/common/orgView/200000888175 -
dc.description AD, Fusion brain organoids, E/I imbalance, Network hyperactivity, PV+ interneuron vulnerability -
dc.description.tableofcontents Ⅰ. Introduction 1
1.1 Genetic and pathological distinctions between sporadic and familial Alzheimer’s Disease 1
1.2 Limitations of traditional AD models and the need for mutation-specific approaches 1
1.3 Establishing human-relevant excitatory and inhibitory circuit models to probe early network dysfunction in AD 3
1.4 Uncovering the role of E/I imbalance and oscillatory deficits in AD through electrophysiological profiling 5
Ⅱ. Materials and Methods 8
2.1 iPSCs lines 8
2.2 Generation of Cx:GE fusion organoid 8
2.3 MEA recording 9
2.4 MEA analysis 10
2.5 Immunohistochemistry 11
2.6 ELISA 12
Ⅲ. Results 13
3.1 Establishing a Cx:GE fusion organoid model for studying E/I network changes in AD 13
3.2 Validation of regional patterning, interneuron migration, and synaptic integration in Cx:GE fusion organoids 17
3.3 Mutation-Specific Mechanisms Underlying Organoid Size Alterations 22
3.4 Region-specific MEA recording and pharmacological dissection of inhibitory and excitatory activity 26
3.5 Dissecting inhibitory tone, excitatory activity, and network activity across AD fusion organoids 29
3.6 Temporal and line-specific alterations in network synchrony in AD fusion organoids 35
3.7 Assessment of network oscillation changes in AD fusion organoids using interspike interval (ISI) coefficient of variation (CoV) 49
3.8 Analysis of theta and gamma rhythms derived from LFP recordings 43
3.9 Temporal progression of AD-related pathology in fusion organoids 48
3.10 Selective vulnerability of PV+ interneurons in AD fusion organoids 53
Ⅳ. Discussion 58
Ⅴ. References 65
Abstract in Korean 71		 -
dc.format.extent 71 -
dc.language eng -
dc.publisher DGIST -
dc.title Investigating the excitatory-inhibitory imbalance in Alzheimer's disease using cerebral cortex and ganglionic eminence fusion organoids -
dc.title.alternative 대뇌 피질-신경절 융기 융합 오가노이드를 활용한 알츠하이머병에서의 흥분-억제 불균형 연구 -
dc.type Thesis -
dc.identifier.doi 10.22677/THESIS.200000888175 -
dc.description.degree Master -
dc.contributor.department Department of Brain Sciences -
dc.contributor.coadvisor Jinsoo Seo -
dc.date.awarded 2025-08-01 -
dc.publisher.location Daegu -
dc.description.database dCollection -
dc.citation XT.BM 최76 202508 -
dc.date.accepted 2025-07-21 -
dc.contributor.alternativeDepartment 뇌과학과 -
dc.subject.keyword AD, Fusion brain organoids, E/I imbalance, Network hyperactivity, PV+ interneuron vulnerability -
dc.contributor.affiliatedAuthor Juran Choe -
dc.contributor.affiliatedAuthor Sung Bae Lee -
dc.contributor.affiliatedAuthor Jinsoo Seo -
dc.contributor.alternativeName 최주란 -
dc.contributor.alternativeName Sung Bae Lee -
dc.contributor.alternativeName 서진수 -
dc.rights.embargoReleaseDate 2029-08-31 -
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