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  Department of New Biology Theses Master

Label-Free and Non-Invasive Classification of Marine Microalgae via Fluorescence Lifetime Imaging Microscopy

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dc.contributor.advisor 이종찬 -
dc.contributor.author Taebin Park -
dc.date.accessioned 2026-01-23T11:01:29Z -
dc.date.available 2026-01-23T11:01:29Z -
dc.date.issued 2025 -
dc.identifier.uri https://scholar.dgist.ac.kr/handle/20.500.11750/59816 -
dc.identifier.uri http://dgist.dcollection.net/common/orgView/200000888214 -
dc.description Fluorescence lifetime imaging microscopy (FLIM), Marine microalgae, Harmful algal blooms (HABs), Chlorophyll autofluorescence, Endogenous fluorescent metabolic cofactors (NAD(P)H and FAD) -
dc.description.tableofcontents List of Contents

Abstract i
List of contents ii,ⅲ,ⅳ


Ⅰ. Introduction
1.1 Marine Microalgae and Harmful Algal Blooms (HABs) 1
1.1.1 Chattonella antiqua (C. antiqua) 1
1.1.2 Heterocapsa circularisquama (H. circularisquama) 1
1.1.3 Nannochloropsis oculata (N. oculata) 1
1.2 Photosynthesis and Cellular Respiration in Microalgae 2
1.3 Chlorophyll Fluorescence as a Functional Marker of Photosynthetic Activity 2
1.4 NAD(P)H and FAD Fluorescence as Indicators of Cellular Metabolism 3
1.5 FLIM for Non-Invasive Metabolic Profiling 5
1.6 The Fluorescence Lifetime Redox Ratio (FLIRR) 5

ⅠI. Materials and methods
2.1 Sample and preparation 6
2.2 Culture maintenance 6
2.3 The instrumentation and Measurement Techniques 7
2.4 Data analysis and statistical methods 8

ⅠII. Results
3.1 Absorption Properties of Autofluorescence in Marine Microalgae 8
3.2 Chlorophyll fluorescence of microalgae 9
3.3 Endogenous Fluorescence Intensity of Microalgal Species 10
3.4 Fluorescence Lifetime of Auto-fluorescent Pigments in Microalgae· 13
3.5 Metabolic Redox States (NAD(P)H and FAD) in Marine Microalgae 18
3.6 Fluorescence Lifetimes of NAD(P)H and FAD in Marine Microalgae 21
3.7 Detailed Interpretation and Statistical Analysis of FLIRR Values 24
3.8 Species Discrimination Based on Chlorophyll and FAD lifetime 27


ⅠV. Discussion
4.1 Absorption Properties of Photo-Reactive Molecules in Marine Microalgae 28
4.2 Fluorescence Spectra of Chlorophyll in Marine Microalgae 30
4.3 Lifetime Imaging of Endogenous Fluorescence in Marine Microalgae 30
4.4 Amplitude Fraction and Fluorescence Lifetime of Metabolic Cofactors in Marine
Microalgae 32
4.5 Evaluation of FLIRR Values in Marine Microalgae 33
4.6 Lifetime-Based Discrimination of Microalgal Species 34

V. Conclusion		 -
dc.format.extent 42 -
dc.language eng -
dc.publisher DGIST -
dc.title Label-Free and Non-Invasive Classification of Marine Microalgae via Fluorescence Lifetime Imaging Microscopy -
dc.type Thesis -
dc.identifier.doi 10.22677/THESIS.200000888214 -
dc.description.degree Master -
dc.contributor.department Department of New Biology -
dc.contributor.coadvisor Yoori Kim -
dc.date.awarded 2025-08-01 -
dc.publisher.location Daegu -
dc.description.database dCollection -
dc.citation XT.NM 박88 202508 -
dc.date.accepted 2025-07-21 -
dc.contributor.alternativeDepartment 뉴바이올로지학과 -
dc.subject.keyword Fluorescence lifetime imaging microscopy (FLIM), Marine microalgae, Harmful algal blooms (HABs), Chlorophyll autofluorescence, Endogenous fluorescent metabolic cofactors (NAD(P)H and FAD) -
dc.contributor.affiliatedAuthor Taebin Park -
dc.contributor.affiliatedAuthor Jong-Chan Lee -
dc.contributor.affiliatedAuthor Yoori Kim -
dc.contributor.alternativeName 박태빈 -
dc.contributor.alternativeName Jong-Chan Lee -
dc.contributor.alternativeName 김유리 -
dc.rights.embargoReleaseDate 2030-08-31 -
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