Title

Label-Free and Non-Invasive Classification of Marine Microalgae via Fluorescence Lifetime Imaging Microscopy

DGIST Authors

Taebin Park ; Jong-Chan Lee ; Yoori Kim

Advisor

이종찬

Co-Advisor(s)

Yoori Kim

Issued Date

2025

Awarded Date

2025-08-01

Type

Thesis

Description

Fluorescence lifetime imaging microscopy (FLIM), Marine microalgae, Harmful algal blooms (HABs), Chlorophyll autofluorescence, Endogenous fluorescent metabolic cofactors (NAD(P)H and FAD)

Table Of Contents

List of Contents

Abstract i
List of contents ii,ⅲ,ⅳ


Ⅰ. Introduction
1.1 Marine Microalgae and Harmful Algal Blooms (HABs) 1
1.1.1 Chattonella antiqua (C. antiqua) 1
1.1.2 Heterocapsa circularisquama (H. circularisquama) 1
1.1.3 Nannochloropsis oculata (N. oculata) 1
1.2 Photosynthesis and Cellular Respiration in Microalgae 2
1.3 Chlorophyll Fluorescence as a Functional Marker of Photosynthetic Activity 2
1.4 NAD(P)H and FAD Fluorescence as Indicators of Cellular Metabolism 3
1.5 FLIM for Non-Invasive Metabolic Profiling 5
1.6 The Fluorescence Lifetime Redox Ratio (FLIRR) 5

ⅠI. Materials and methods
2.1 Sample and preparation 6
2.2 Culture maintenance 6
2.3 The instrumentation and Measurement Techniques 7
2.4 Data analysis and statistical methods 8

ⅠII. Results
3.1 Absorption Properties of Autofluorescence in Marine Microalgae 8
3.2 Chlorophyll fluorescence of microalgae 9
3.3 Endogenous Fluorescence Intensity of Microalgal Species 10
3.4 Fluorescence Lifetime of Auto-fluorescent Pigments in Microalgae· 13
3.5 Metabolic Redox States (NAD(P)H and FAD) in Marine Microalgae 18
3.6 Fluorescence Lifetimes of NAD(P)H and FAD in Marine Microalgae 21
3.7 Detailed Interpretation and Statistical Analysis of FLIRR Values 24
3.8 Species Discrimination Based on Chlorophyll and FAD lifetime 27


ⅠV. Discussion
4.1 Absorption Properties of Photo-Reactive Molecules in Marine Microalgae 28
4.2 Fluorescence Spectra of Chlorophyll in Marine Microalgae 30
4.3 Lifetime Imaging of Endogenous Fluorescence in Marine Microalgae 30
4.4 Amplitude Fraction and Fluorescence Lifetime of Metabolic Cofactors in Marine
Microalgae 32
4.5 Evaluation of FLIRR Values in Marine Microalgae 33
4.6 Lifetime-Based Discrimination of Microalgal Species 34

V. Conclusion

URI

https://scholar.dgist.ac.kr/handle/20.500.11750/59816
http://dgist.dcollection.net/common/orgView/200000888214

DOI

10.22677/THESIS.200000888214

Degree

Master

Department

Department of New Biology

Publisher

DGIST

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