Title

Sub-dose combination of R406 and ABT-263 enhances senolysis via ROS generation

Alternative Title

활성산소종(ROS) 생성을 통한 R406 및 ABT-263 저용량 병용의 향상된 노화세포 제거 효과

DGIST Authors

Ahyeon Yun ; Young-Sam Lee ; Yun-Il Lee

Advisor

이영삼

Co-Advisor(s)

Yun-Il Lee

Issued Date

2025

Awarded Date

2025-08-01

Type

Thesis

Description

Aging, Cellular senescence, Senolytics, Synergism, FAK, Bcl-2

Table Of Contents

List of Contents

Abstract i
List of contents ii-ⅲ
List of figures vi

Ⅰ. Introduction
1.1. Aging and Cellular Senescence 1
1.2. Age-related Diseases Associated With Cellular Senescence 2
1.3. Senotherapies: Senolytics and Senomorphics 3
1.4. Combination Therapy and Synergism 4
1.5. Synergyfinder: A Tool For Evaluating Synergistic Effect 5
1.6. R406 6
1.7. ABT-263 7
1.8. Overview of This Study 8

Ⅱ. Experimental Method & Materials
2.1. Cell Culture 9
2.2. Drug preparation 9
2.3. Cell viability assay 10
2.4. Flow cytometry 10
2.5. Synergyfinder 10
2.6. Western blot 11
2.7. ROS assay 11
2.8. Animals 12
2.9. Immunohistochemical staining 12
2.10. Statistical analyses 13

Ⅲ. Results
3.1.Identification of R406 and ABT-263 as a novel senolytic combination 14
3.2.In vitro Synergy Analysis of R406 and ABT-263 for Senolytic effect 17
3.3.Non-apoptotic cell death by sub-dose co-treatment of R406 and ABT-263 26
3.4.ROS-mediated senolysis by the combination of R406 and ABT-263 29
3.5.In vivo evaluation in a bleomycin-induced pulmonary fibrosis model 35

Ⅳ. Discussion
43

Ⅴ. Conclusion
46

Ⅵ. References
47

요 약 문
53

URI

https://scholar.dgist.ac.kr/handle/20.500.11750/59818
http://dgist.dcollection.net/common/orgView/200000889847

DOI

10.22677/THESIS.200000889847

Degree

Master

Department

Department of New Biology

Publisher

DGIST

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