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  Department of Brain Sciences Theses Master

Identification of intrinsic resilience factors in MNs to ALS-toxicity

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dc.contributor.advisor 백명인 -
dc.contributor.author Jiyoung Park -
dc.date.accessioned 2026-09-01T19:30:59Z -
dc.date.available 2026-09-01T19:30:59Z -
dc.date.issued 2026 -
dc.identifier.uri https://scholar.dgist.ac.kr/handle/20.500.11750/60764 -
dc.identifier.uri http://dgist.dcollection.net/common/orgView/200001007748 -
dc.description Amyotrophic lateral sclerosis, TDP-43 proteinopathy, chicken embryo, spinal cord, RNA-sequencing -
dc.description.tableofcontents List of Contents
Abstract i
List of contents ii
List of tables iv
List of figures v

Ⅰ. Introduction
1.1. Overview of ALS 1
1.2. TDP-43 pathology in ALS 1
1.3. TDP-43 Q331K 3
1.4. Current therapeutic limitations in ALS 4
1.5. Selective vulnerability and resilience of MNs in ALS 4
1.6. Chick embryo model 5
1.7. Study rationale and objective 5

II. Materials and Methods
2.1. Egg preparation 7
2.2. In ovo electroporation of chick embryos 7
2.3. Cell isolation 8
2.4. cDNA synthesis and pre-amplification 8
2.5. Quantitative real-time PCR 9
2.6. Gene synthesis and cloning 10
2.7. RNA-sequencing 11
2.8. Tissue collection 11
2.9. Immunohistochemistry 11
2.10. RNA in situ hybridization 12

III. Results
3.1. Establishment of a chick embryonic spinal cord model for TDP-43 Q331K-induced toxicity 14
3.2. Transcriptome analysis identifies candidate genes in Hb9-GFP-positive MNs under TDP-43 Q331K-induced toxicity 17
3.3. RNA in situ hybridization analysis of candidate genes in the chick embryonic spinal cord 20
3.4. Preliminary analysis of time-dependent trends in TDP-43 Q331K subcellular localization 22
3.5. Analysis of the effect of LOC430766 co-expression on TDP-43-positive cell number and subcellular localization 25
3.6. Analysis of the effect of ULK1 co-expression on TDP-43-positive cell number and subcellular localization 29
3.7. Optimization of an mRNA-based TDP-43 Q331K expression system 33

IV. Discussion 37
V. References 47
요약문 54		 -
dc.format.extent 54 -
dc.language eng -
dc.publisher DGIST -
dc.title Identification of intrinsic resilience factors in MNs to ALS-toxicity -
dc.type Thesis -
dc.identifier.doi 10.22677/THESIS.200001007748 -
dc.description.degree Master -
dc.contributor.department Department of Brain Sciences -
dc.contributor.coadvisor Byung-Chang Suh -
dc.date.awarded 2026-08-01 -
dc.publisher.location Daegu -
dc.description.database dCollection -
dc.citation XT.BM 박78 202608 -
dc.date.accepted 2026-07-21 -
dc.contributor.alternativeDepartment 뇌과학과 -
dc.subject.keyword Amyotrophic lateral sclerosis, TDP-43 proteinopathy, chicken embryo, spinal cord, RNA-sequencing -
dc.contributor.affiliatedAuthor Jiyoung Park -
dc.contributor.affiliatedAuthor Myungin Baek -
dc.contributor.affiliatedAuthor Byung-Chang Suh -
dc.contributor.alternativeName 박지영 -
dc.contributor.alternativeName Myungin Baek -
dc.contributor.alternativeName 서병창 -
dc.rights.embargoReleaseDate 2031-08-31 -
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