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Olfactory marker protein regulates prolactin secretion and production by modulating Ca2+ and TRH signaling in lactotrophs

Title
Olfactory marker protein regulates prolactin secretion and production by modulating Ca2+ and TRH signaling in lactotrophs
Authors
Kang, Chan WooHan, Ye EonLee, Mi KyungCho, Yoon HeeKang, Na NaKoo, Jae HyungKu, Cheol RyongLee, Eun Jig
DGIST Authors
Kang, Na Na; Koo, Jae Hyung
Issue Date
2018-04
Citation
Experimental and Molecular Medicine, 50(4), 1-11
Type
Article
Article Type
Article
Keywords
THYROTROPIN-RELEASING-HORMONE; VECTOR-MEDIATED RESCUE; PITUITARY-CELLS; GENE-EXPRESSION; NEUROENDOCRINE CARCINOMAS; ADENYLYL-CYCLASE; RECEPTOR; MOUSE; OMP; KINASE
ISSN
1226-3613
Abstract
Olfactory marker protein (OMP) is a marker of olfactory receptor-mediated chemoreception, even outside the olfactory system. Here, we report that OMP expression in the pituitary gland plays a role in basal and thyrotropin-releasing hormone (TRH)-induced prolactin (PRL) production and secretion. We found that OMP was expressed in human and rodent pituitary glands, especially in PRL-secreting lactotrophs. OMP knockdown in GH4 rat pituitary cells increased PRL production and secretion via extracellular signal-regulated kinase (ERK)1/2 signaling. Real-time PCR analysis and the Ca2+ influx assay revealed that OMP was critical for TRH-induced PRL secretion. OMP-knockout mice showed lower fertility than control mice, which was associated with increased basal PRL production via activation of ERK1/2 signaling and reduced TRH-induced PRL secretion. However, both in vitro and in vivo results indicated that OMP was only required for hormone production and secretion because ERK1/2 activation failed to stimulate cell proliferation. Additionally, patients with prolactinoma lacked OMP expression in tumor tissues with hyperactivated ERK1/2 signaling. These findings indicate that OMP plays a role in PRL production and secretion in lactotrophs through the modulation of Ca2+ and TRH signaling. © 2018 The Author(s).
URI
http://hdl.handle.net/20.500.11750/6249
DOI
10.1038/s12276-018-0035-z
Publisher
Nature Publishing Group
Related Researcher
  • Author Koo, JaeHyung Molecular & Cellular Smell Biology Lab
  • Research Interests Neurotranslation and chemosensation; 화학감각신경(후각, 미각) 및 이소성후각수용체 연구와 응용
Files:
Collection:
ETC1. Journal Articles
Department of New BiologyMolecular & Cellular Smell Biology Lab1. Journal Articles


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