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Pumpless Microflow Cytometry Enabled by Viscosity Modulation and Immunobead Labeling
- Pumpless Microflow Cytometry Enabled by Viscosity Modulation and Immunobead Labeling
- Kim, Byeong Yeon; Oh, Se In; Shin, Su Yeon; Yim, Sang Gu; Yang, Seung Yun; Hahn, Young Ki; Choi, Sung Young
- Issue Date
- Analytical Chemistry, 90(13), 8254-8260
- Article Type
- CIRCULATING TUMOR-CELLS; FLOW-CYTOMETRY; STEM-CELLS; SHEATHLESS; SORTER
- Major challenges of miniaturizing flow cytometry include obviating the need for bulky, expensive, and complex pump-based fluidic and laser-based optical systems while retaining the ability to detect target cells based on their unique surface receptors. We addressed these critical challenges by (i) using a viscous liquid additive to control flow rate passively, without external pumping equipment, and (ii) adopting an immunobead assay that can be quantified with a portable fluorescence cell counter based on a blue light-emitting diode. Such novel features enable pumpless microflow cytometry (pFC) analysis by simply dropping a sample solution onto the inlet reservoir of a disposable cell-counting chamber. With our pFC platform, we achieved reliable cell counting over a dynamic range of 9-298 cells/μL. We demonstrated the practical utility of the platform by identifying a type of cancer cell based on CD326, the epithelial cell adhesion molecule. This portable microflow cytometry platform can be applied generally to a range of cell types using immunobeads labeled with specific antibodies, thus making it valuable for cell-based and point-of-care diagnostics. Copyright © 2018 American Chemical Society.
- American Chemical Society
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