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Pumpless Microflow Cytometry Enabled by Viscosity Modulation and Immunobead Labeling

Title
Pumpless Microflow Cytometry Enabled by Viscosity Modulation and Immunobead Labeling
Authors
Kim, Byeong YeonOh, Se InShin, Su YeonYim, Sang GuYang, Seung YunHahn, Young KiChoi, Sung Young
Issue Date
2018-07
Citation
Analytical Chemistry, 90(13), 8254-8260
Type
Article
Article Type
Article
Keywords
CIRCULATING TUMOR-CELLSFLOW-CYTOMETRYSTEM-CELLSSHEATHLESSSORTER
ISSN
0003-2700
Abstract
Major challenges of miniaturizing flow cytometry include obviating the need for bulky, expensive, and complex pump-based fluidic and laser-based optical systems while retaining the ability to detect target cells based on their unique surface receptors. We addressed these critical challenges by (i) using a viscous liquid additive to control flow rate passively, without external pumping equipment, and (ii) adopting an immunobead assay that can be quantified with a portable fluorescence cell counter based on a blue light-emitting diode. Such novel features enable pumpless microflow cytometry (pFC) analysis by simply dropping a sample solution onto the inlet reservoir of a disposable cell-counting chamber. With our pFC platform, we achieved reliable cell counting over a dynamic range of 9-298 cells/μL. We demonstrated the practical utility of the platform by identifying a type of cancer cell based on CD326, the epithelial cell adhesion molecule. This portable microflow cytometry platform can be applied generally to a range of cell types using immunobeads labeled with specific antibodies, thus making it valuable for cell-based and point-of-care diagnostics. Copyright © 2018 American Chemical Society.
URI
http://hdl.handle.net/20.500.11750/9046
DOI
10.1021/acs.analchem.8b01804
Publisher
American Chemical Society
Files:
There are no files associated with this item.
Collection:
ETC1. Journal Articles


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