Cited 2 time in webofscience Cited 6 time in scopus

A secretome profile indicative of oleate-induced proliferation of HepG2 hepatocellular carcinoma cells

Title
A secretome profile indicative of oleate-induced proliferation of HepG2 hepatocellular carcinoma cells
Authors
Park, SoyeonPark, Ji-HwanJung, Hee-JungJang, Jin-HyeokAhn, SanghyunKim, YounahSuh, Pann-GhillChae, SehyunYoon, Jong HyukRyu, Sung HoHwang, Daehee
DGIST Authors
Jang, Jin-Hyeok; Hwang, Daehee
Issue Date
2018-08
Citation
Experimental and Molecular Medicine, 50(8), 1-14
Type
Article
Article Type
Article
Keywords
ENDOPLASMIC-RETICULUM-STRESSMIGRATION INHIBITORY FACTORUNSATURATED FATTY-ACIDSBREAST-CANCER CELLSCANDIDATE SEROLOGICAL BIOMARKERSTHIOL OXIDOREDUCTASE ERP57UNFOLDED PROTEIN RESPONSEPANCREATIC-CANCERMASS-SPECTROMETRYINDUCED APOPTOSIS
ISSN
1226-3613
Abstract
Increased fatty acid (FA) is often observed in highly proliferative tumors. FAs have been shown to modulate the secretion of proteins from tumor cells, contributing to tumor survival. However, the secreted factors affected by FA have not been systematically explored. Here, we found that treatment of oleate, a monounsaturated omega-9 FA, promoted the proliferation of HepG2 cells. To examine the secreted factors associated with oleate-induced cell proliferation, we performed a comprehensive secretome profiling of oleate-treated and untreated HepG2 cells. A comparison of the secretomes identified 349 differentially secreted proteins (DSPs; 145 upregulated and 192 downregulated) in oleate-treated samples, compared to untreated samples. The functional enrichment and network analyses of the DSPs revealed that the 145 upregulated secreted proteins by oleate treatment were mainly associated with cell proliferation-related processes, such as lipid metabolism, inflammatory response, and ER stress. Based on the network models of the DSPs, we selected six DSPs (MIF, THBS1, PDIA3, APOA1, FASN, and EEF2) that can represent such processes related to cell proliferation. Thus, our results provided a secretome profile indicative of an oleate-induced proliferation of HepG2 cells. © 2018, The Author(s).
URI
http://hdl.handle.net/20.500.11750/9231
DOI
10.1038/s12276-018-0120-3
Publisher
Nature Publishing Group
Files:
Collection:
ETC1. Journal Articles
Department of New BiologySystems Biology and Medicine Lab1. Journal Articles


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