Cited 4 time in webofscience Cited 3 time in scopus

Effect of young exosomes injected in aged mice

Title
Effect of young exosomes injected in aged mice
Authors
Lee, Bo-RamKim, Jung-HeeChoi, Eun-SookCho, Jung-HoonKim, Eunjoo
DGIST Authors
Lee, Bo-Ram; Kim, Jung-Hee; Kim, Eunjoo
Issue Date
2018-09
Citation
International journal of nanomedicine, 13, 5335-5345
Type
Article
Article Type
Article
Keywords
exosomeinjectionreverse agingtelomerasebiomarkermolecular therapyCELLSEXPRESSIONMIRNADISEASEBLOODTELOMERASESENESCENCEMICRORNASBIOMARKERPATHWAYS
ISSN
1178-2013
Abstract
Introduction: Exosomes, nano-sized extracellular vesicles, are known to circulate through the blood stream to transfer molecular signals from tissue to tissue. Methods: To determine whether exosomes affect aging in animals, we primarily identified the changes in exosomal miRNA contents during the aging process. In exosomes from 12-month-old mice, mmu-miR-126-5p and mmu-miR-466c-5p levels were decreased and mmu-miR-184-3p and mmu-miR-200b-5p levels were increased significantly compared with those of 3-month-old mice. Their levels in exosomes were partially correlated with those in tissues: levels of only mmu-miR-126-5p and mmu-miR-466c-5p in lungs and/or liver were decreased, but those of mmu-miR-184-3p and mmu-miR-200b-5p in tissues did not coincide with those of exosomes. Results and discussion: In the aged tissues injected with young exosomes isolated from serum, mmu-miR-126b-5p levels were reversed in the lungs and liver. Expression changes in aging-associated molecules in young exosome-injected mice were obvious: p16(Ink4A), MTOR, and IGF1R were significantly downregulated in the lungs and/or liver of old mice. In addition, telomerase-related genes such as Men1, Mre11a, Tep1, Terf2, Tert, and Tnks were significantly upregulated in the liver of old mice after injection of young exosomes. Conclusion: These results indicate that exosomes from young mice could reverse the expression pattern of aging-associated molecules in aged mice. Eventually, exosomes may be used as a novel approach for the treatment and diagnosis of aging animals.
URI
http://hdl.handle.net/20.500.11750/9348
DOI
10.2147/IJN.S170680
Publisher
Dove Medical Press Ltd
Related Researcher
  • Author Kim, Eunjoo  
  • Research Interests Biomarker, liquid biopsy, nanomedicine, molecular diagnosis
Files:
There are no files associated with this item.
Collection:
Companion Diagnostics and Medical Technology Research Group1. Journal Articles


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