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Effect of young exosomes injected in aged mice
- Effect of young exosomes injected in aged mice
- Lee, Bo-Ram; Kim, Jung-Hee; Choi, Eun-Sook; Cho, Jung-Hoon; Kim, Eunjoo
- DGIST Authors
- Lee, Bo-Ram; Kim, Jung-Hee; Kim, Eunjoo
- Issue Date
- International journal of nanomedicine, 13, 5335-5345
- Article Type
- exosome; injection; reverse aging; telomerase; biomarker; molecular therapy; CELLS; EXPRESSION; MIRNA; DISEASE; BLOOD; TELOMERASE; SENESCENCE; MICRORNAS; BIOMARKER; PATHWAYS
- Introduction: Exosomes, nano-sized extracellular vesicles, are known to circulate through the blood stream to transfer molecular signals from tissue to tissue. Methods: To determine whether exosomes affect aging in animals, we primarily identified the changes in exosomal miRNA contents during the aging process. In exosomes from 12-month-old mice, mmu-miR-126-5p and mmu-miR-466c-5p levels were decreased and mmu-miR-184-3p and mmu-miR-200b-5p levels were increased significantly compared with those of 3-month-old mice. Their levels in exosomes were partially correlated with those in tissues: levels of only mmu-miR-126-5p and mmu-miR-466c-5p in lungs and/or liver were decreased, but those of mmu-miR-184-3p and mmu-miR-200b-5p in tissues did not coincide with those of exosomes. Results and discussion: In the aged tissues injected with young exosomes isolated from serum, mmu-miR-126b-5p levels were reversed in the lungs and liver. Expression changes in aging-associated molecules in young exosome-injected mice were obvious: p16(Ink4A), MTOR, and IGF1R were significantly downregulated in the lungs and/or liver of old mice. In addition, telomerase-related genes such as Men1, Mre11a, Tep1, Terf2, Tert, and Tnks were significantly upregulated in the liver of old mice after injection of young exosomes. Conclusion: These results indicate that exosomes from young mice could reverse the expression pattern of aging-associated molecules in aged mice. Eventually, exosomes may be used as a novel approach for the treatment and diagnosis of aging animals.
- Dove Medical Press Ltd
- Related Researcher
Biomarker, liquid biopsy, nanomedicine, molecular diagnosis
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- Companion Diagnostics and Medical Technology Research Group1. Journal Articles
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