Presenilin-2 N141I Knockin Mice as a Model of Alzheimer’s Disease
- Title
- Presenilin-2 N141I Knockin Mice as a Model of Alzheimer’s Disease
- Author(s)
- Ji-Eun Gim
- DGIST Authors
- Yu, Seong-Woon ; Gim, Ji-Eun ; Lee, Byung-Hoon
- Advisor
- 유성운
- Co-Advisor(s)
- Byung-Hoon Lee
- Issued Date
- 2019
- Awarded Date
- 2019-02
- Type
- Thesis
- Table Of Contents
-
1. Introduction
2. Material and Methods
2.1 Cell culture 7
2.2 Western blotting analysis (WB) 7
2.3 RNA extraction and Quantitative real-time polymerase chain reaction (qRT-PCR) 8
2.4 Reagents and antibodies 11
2.5 CORT measurement 11
2.6 Y-maze test 11
2.7 Statistical analysis 12
3. Results
3.1 Generation of PS2 N141I KI mice 13
3.2 Cognitive decline was detected in PS2 N141I KI mice 15
3.3 PS2 CTF levels are decreased in PS2 N141I KI mice 19
3.4 CORT treatment increased PS2 level in HCN cells 21
3.4 PS2 expression in CORT treated HCN cells is regulated by autophagy 23
4. Discussion
Reference
Abstract in Korean
- URI
-
http://dgist.dcollection.net/common/orgView/200000171529
http://hdl.handle.net/20.500.11750/10698
- Degree
- MASTER
- Department
- Brain and Cognitive Sciences
- Publisher
- DGIST
- Related Researcher
-
-
Yu, Seong-Woon
- Research Interests Molecular mechanisms of neuronal cell death and neurodegeneration
-
- Files in This Item:
-
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- Appears in Collections:
- Department of Brain Sciences Theses Master
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