Title

Presenilin-2 N141I Knockin Mice as a Model of Alzheimer’s Disease

DGIST Authors

Yu, Seong-Woon ; Gim, Ji-Eun ; Lee, Byung-Hoon

Advisor

유성운

Co-Advisor(s)

Byung-Hoon Lee

Issued Date

2019

Awarded Date

2019-02

Citation

Ji-Eun Gim. (2019). Presenilin-2 N141I Knockin Mice as a Model of Alzheimer’s Disease. doi: 10.22677/thesis.200000171529

Type

Thesis

Table Of Contents

1. Introduction
2. Material and Methods
2.1 Cell culture 7
2.2 Western blotting analysis (WB) 7
2.3 RNA extraction and Quantitative real-time polymerase chain reaction (qRT-PCR) 8
2.4 Reagents and antibodies 11
2.5 CORT measurement 11
2.6 Y-maze test 11
2.7 Statistical analysis 12

3. Results
3.1 Generation of PS2 N141I KI mice 13
3.2 Cognitive decline was detected in PS2 N141I KI mice 15
3.3 PS2 CTF levels are decreased in PS2 N141I KI mice 19
3.4 CORT treatment increased PS2 level in HCN cells 21
3.4 PS2 expression in CORT treated HCN cells is regulated by autophagy 23

4. Discussion
Reference
Abstract in Korean

URI

http://dgist.dcollection.net/common/orgView/200000171529
http://hdl.handle.net/20.500.11750/10698

DOI

10.22677/thesis.200000171529

Degree

MASTER

Department

Brain and Cognitive Sciences

Publisher

DGIST

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