Cited 3 time in webofscience Cited 5 time in scopus

The small GTPase ARF6 regulates GABAergic synapse development

Title
The small GTPase ARF6 regulates GABAergic synapse development
Authors
Kim, HyeonhoJung, HyejiJung, HyunsuKwon, Seok-KyuKo, JaewonUm, Ji Won
DGIST Authors
Ko, JaewonUm, Ji Won
Issue Date
2020-01
Citation
Molecular Brain, 13(1), 2
Type
Article
Article Type
Article
Author Keywords
ARFEpilepsyGABAInhibitory synapse
Keywords
NUCLEOTIDE EXCHANGE FACTORPOSTSYNAPTIC DENSITYPRESYNAPTIC ROLEG-PROTEINSFAMILYMATURATIONMSEC7-1EFA6ABRAG1GIT1
ISSN
1756-6606
Abstract
ADP ribosylation factors (ARFs) are a family of small GTPases composed of six members (ARF1-6) that control various cellular functions, including membrane trafficking and actin cytoskeletal rearrangement, in eukaryotic cells. Among them, ARF1 and ARF6 are the most studied in neurons, particularly at glutamatergic synapses, but their roles at GABAergic synapses have not been investigated. Here, we show that a subset of ARF6 protein is localized at GABAergic synapses in cultured hippocampal neurons. In addition, we found that knockdown (KD) of ARF6, but not ARF1, triggered a reduction in the number of GABAergic synaptic puncta in mature cultured neurons in an ARF activity-dependent manner. ARF6 KD also reduced GABAergic synaptic density in the mouse hippocampal dentate gyrus (DG) region. Furthermore, ARF6 KD in the DG increased seizure susceptibility in an induced epilepsy model. Viewed together, our results suggest that modulating ARF6 and its regulators could be a therapeutic strategy against brain pathologies involving hippocampal network dysfunction, such as epilepsy. © 2020 The Author(s).
URI
http://hdl.handle.net/20.500.11750/11420
DOI
10.1186/s13041-019-0543-3
Publisher
BioMed Central
Related Researcher
  • Author Ko, Jaewon Laboratory of Synapse Formation and Function
  • Research Interests Synapse Formation and Function; Neural Circuits; 뇌질환; animal model
Files:
Collection:
Department of Brain and Cognitive SciencesSynapse Disorder Laboratory1. Journal Articles
Department of Brain and Cognitive SciencesLaboratory of Synapse Formation and Function1. Journal Articles


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