Cited 2 time in
Cited 2 time in
Supra-blot: an accurate and reliable assay for detecting target proteins with a synthetic host molecule-enzyme hybrid
- Supra-blot: an accurate and reliable assay for detecting target proteins with a synthetic host molecule-enzyme hybrid
- Sung, Gihyun; Lee, Song-Yi; Kang, Myeong-Gyun; Kim, Kyung Lock; An, Jaeyeon; Sim, Jaehwan; Kim, Sungwan; Kim, Seung Jun; Ko, Jaewon; Rhee, Hyun-Woo; Park, Kyeng Min; Kim, Kimoon
- DGIST Authors
- Ko, Jaewon
- Issue Date
- Chemical Communications, 56(10), 1549-1552
- Article Type
- ENDOGENOUS BIOTIN; STREPTAVIDIN; TAG; TECHNOLOGY; PEROXIDASE; PEPTIDE; AZIDES; CELLS; PAIR
- In accordance with the rapid increase in demand for selective and spatial chemical tagging, and accurate detection of proteins of interest, we develop a sensitive protein detection method, termed "Supra-blot" capitalizing on high-affinity host-guest interaction between cucurbituril (CB) and adamantylammonium (AdA). The method can directly detect chemically tagged proteins without false-positive signals caused by endogenous biomolecules. Not only a single specific protein, but also spatially localized proteins in cells were labeled with AdA, and selectively detected by a host molecule-enzyme hybrid, CB-conjugated horseradish peroxidase (CB-HRP) generating amplified chemiluminescence signals. This study shows the great potential of Supra-blot for accurate and reliable detection of proteins of interest in cells. © 2020 The Royal Society of Chemistry.
- Royal Society of Chemistry
- Related Researcher
Laboratory of Synapse Formation and Function
Synapse Formation and Function; Neural Circuits; 뇌질환; animal model
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- Department of Brain and Cognitive SciencesLaboratory of Synapse Formation and Function1. Journal Articles
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