Cited 2 time in webofscience Cited 2 time in scopus

Supra-blot: an accurate and reliable assay for detecting target proteins with a synthetic host molecule-enzyme hybrid

Title
Supra-blot: an accurate and reliable assay for detecting target proteins with a synthetic host molecule-enzyme hybrid
Authors
Sung, GihyunLee, Song-YiKang, Myeong-GyunKim, Kyung LockAn, JaeyeonSim, JaehwanKim, SungwanKim, Seung JunKo, JaewonRhee, Hyun-WooPark, Kyeng MinKim, Kimoon
DGIST Authors
Ko, Jaewon
Issue Date
2020-02
Citation
Chemical Communications, 56(10), 1549-1552
Type
Article
Article Type
Article
Keywords
ENDOGENOUS BIOTINSTREPTAVIDINTAGTECHNOLOGYPEROXIDASEPEPTIDEAZIDESCELLSPAIR
ISSN
1359-7345
Abstract
In accordance with the rapid increase in demand for selective and spatial chemical tagging, and accurate detection of proteins of interest, we develop a sensitive protein detection method, termed "Supra-blot" capitalizing on high-affinity host-guest interaction between cucurbit[7]uril (CB[7]) and adamantylammonium (AdA). The method can directly detect chemically tagged proteins without false-positive signals caused by endogenous biomolecules. Not only a single specific protein, but also spatially localized proteins in cells were labeled with AdA, and selectively detected by a host molecule-enzyme hybrid, CB[7]-conjugated horseradish peroxidase (CB[7]-HRP) generating amplified chemiluminescence signals. This study shows the great potential of Supra-blot for accurate and reliable detection of proteins of interest in cells. © 2020 The Royal Society of Chemistry.
URI
http://hdl.handle.net/20.500.11750/11518
DOI
10.1039/C9CC09699J
Publisher
Royal Society of Chemistry
Related Researcher
  • Author Ko, Jaewon Laboratory of Synapse Formation and Function
  • Research Interests Synapse Formation and Function; Neural Circuits; 뇌질환; animal model
Files:
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Collection:
Department of Brain and Cognitive SciencesLaboratory of Synapse Formation and Function1. Journal Articles


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