Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Lee, Tae Hee | ko |
dc.contributor.author | Kim, Kwang Soo | ko |
dc.contributor.author | Kim, Jin Hee | ko |
dc.contributor.author | Jeong, Jae-Ho | ko |
dc.contributor.author | Woo, Hye Ryun | ko |
dc.contributor.author | Park, So Ra | ko |
dc.contributor.author | Sohn, Myung-Ho | ko |
dc.contributor.author | Lee, Hyeon Ju | ko |
dc.contributor.author | Rhee, Joon Haeng | ko |
dc.contributor.author | Cha, Sun-Shin | ko |
dc.contributor.author | Hwang, Joo-Hee | ko |
dc.contributor.author | Chung, Kyung Min | ko |
dc.date.accessioned | 2020-04-02T10:16:37Z | - |
dc.date.available | 2020-04-02T10:16:37Z | - |
dc.date.created | 2020-03-03 | - |
dc.date.issued | 2020-04 | - |
dc.identifier.citation | Journal of Immunological Methods, v.479, pp.112750 | - |
dc.identifier.issn | 0022-1759 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/11614 | - |
dc.description.abstract | The specific recognition between a monoclonal antibody (mAb) and its epitope can be used in a tag system that has proved valuable in a wide range of biological applications. Herein, we describe a novel tag called RA-tag that is composed of a seven amino acid sequence (DIDLSRI) and recognized by a highly specific mAb, 47RA, against the bacterial toxin Vibrio vulnificus RtxA1/MARTXVv. By using recombinant proteins with the RA-tag at the N-terminal, C-terminal, or an internal site, we demonstrated that the tag system could be an excellent biological system for both protein purification and protein detection in enzyme-linked immunosorbent, Western blot, flow cytometry, and immunofluorescence staining analyses in Escherichia coli, mammalian cell lines, yeast, and plant. In addition, our RA-tag/47RA mAb combination showed high sensitivity and reliable affinity (KD = 5.90 × 10−8 M) when compared with conventional tags. Overall, our results suggest that the RA-tag system could facilitate the development of a broadly applicable tag system for biological research. © 2020 | - |
dc.language | English | - |
dc.publisher | Elsevier BV | - |
dc.title | Novel short peptide tag from a bacterial toxin for versatile applications | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.jim.2020.112750 | - |
dc.identifier.wosid | 000520019300006 | - |
dc.identifier.scopusid | 2-s2.0-85078452386 | - |
dc.type.local | Article(Overseas) | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.contributor.nonIdAuthor | Lee, Tae Hee | - |
dc.contributor.nonIdAuthor | Kim, Kwang Soo | - |
dc.contributor.nonIdAuthor | Jeong, Jae-Ho | - |
dc.contributor.nonIdAuthor | Park, So Ra | - |
dc.contributor.nonIdAuthor | Sohn, Myung-Ho | - |
dc.contributor.nonIdAuthor | Lee, Hyeon Ju | - |
dc.contributor.nonIdAuthor | Rhee, Joon Haeng | - |
dc.contributor.nonIdAuthor | Cha, Sun-Shin | - |
dc.contributor.nonIdAuthor | Hwang, Joo-Hee | - |
dc.contributor.nonIdAuthor | Chung, Kyung Min | - |
dc.identifier.citationVolume | 479 | - |
dc.identifier.citationStartPage | 112750 | - |
dc.identifier.citationTitle | Journal of Immunological Methods | - |
dc.type.journalArticle | Article | - |
dc.description.isOpenAccess | N | - |
dc.subject.keywordAuthor | Epitope tag | - |
dc.subject.keywordAuthor | Monoclonal antibody | - |
dc.subject.keywordAuthor | Immunodetection | - |
dc.subject.keywordAuthor | Affinity purification | - |
dc.subject.keywordPlus | RECOMBINANT PROTEIN EXPRESSION | - |
dc.subject.keywordPlus | MYC ANTIBODY 9E10 | - |
dc.subject.keywordPlus | MONOCLONAL-ANTIBODIES | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | SUBCELLULAR-LOCALIZATION | - |
dc.subject.keywordPlus | STEP PURIFICATION | - |
dc.subject.keywordPlus | AFFINITY PEPTIDE | - |
dc.subject.keywordPlus | HNRNP A1 | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.contributor.affiliatedAuthor | Woo, Hye Ryun | - |
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