Title

CK2-mediated phosphorylation of caspase-9 switches autophagic cell death to apoptosis in adult hippocampal neural stem cells following corticosterone treatment.

DGIST Authors

Jeong, Youngtae ; Lee, Ye Won ; Yu, Seong-Woon

Advisor

유성운

Co-Advisor(s)

Youngtae Jeong

Issued Date

2020

Awarded Date

2020-02

Citation

Ye Won Lee. (2020). CK2-mediated phosphorylation of caspase-9 switches autophagic cell death to apoptosis in adult hippocampal neural stem cells following corticosterone treatment. doi: 10.22677/Theses.200000286669

Type

Thesis

Description

Autophagic cell death (ACD), Apoptosis, Corticosterone, Hippocampal neural stem (HCN) cells, Casein kinase 2 (CK 2), Caspase 9

Table Of Contents

Ⅰ. Introduction 1
Ⅱ. Material and Methods 11
2.1 Reagents and antibodies 11
2.2 Cell culture 11
2.3 Transfection, Expression vectors 12
2.4 Cell death assay 13
2.5 Western blotting 13
2.6 Caspase-9 activity assay 15
2.7 Immunocytochemistry 16
2.8 Live cell imaging 16
2.9 Statistical analysis 17

Ⅲ. Results 18
3.1 CASP9 is activated upon CORT-induced ACD in HCN cells. 18
3.2 Genetic ablation of CASP9 impairs CORT-induced ACD. 21
3.3 Regulation of caspase-9 by phosphorylation 23
3.4 CK2 inhibition switches ACD to apoptosis in HCN cells. 25
3.5 The cell death switching is dependent on ACD. 28

Ⅳ. Discussion 31
Ⅴ. Reference 34
Abstract in Korean 39

URI

http://dgist.dcollection.net/common/orgView/200000281857
http://hdl.handle.net/20.500.11750/11915

DOI

10.22677/Theses.200000286669

Degree

Master

Department

Brain and Cognitive Sciences

Publisher

DGIST

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