CK2-mediated phosphorylation of caspase-9 switches autophagic cell death to apoptosis in adult hippocampal neural stem cells following corticosterone treatment.
- Title
- CK2-mediated phosphorylation of caspase-9 switches autophagic cell death to apoptosis in adult hippocampal neural stem cells following corticosterone treatment.
- Author(s)
- Ye Won Lee
- DGIST Authors
- Jeong, Youngtae ; Lee, Ye Won ; Yu, Seong-Woon
- Advisor
- 유성운
- Co-Advisor(s)
- Youngtae Jeong
- Issued Date
- 2020
- Awarded Date
- 2020-02
- Type
- Thesis
- Description
- Autophagic cell death (ACD), Apoptosis, Corticosterone, Hippocampal neural stem (HCN) cells, Casein kinase 2 (CK 2), Caspase 9
- Table Of Contents
-
Ⅰ. Introduction 1
Ⅱ. Material and Methods 11
2.1 Reagents and antibodies 11
2.2 Cell culture 11
2.3 Transfection, Expression vectors 12
2.4 Cell death assay 13
2.5 Western blotting 13
2.6 Caspase-9 activity assay 15
2.7 Immunocytochemistry 16
2.8 Live cell imaging 16
2.9 Statistical analysis 17
Ⅲ. Results 18
3.1 CASP9 is activated upon CORT-induced ACD in HCN cells. 18
3.2 Genetic ablation of CASP9 impairs CORT-induced ACD. 21
3.3 Regulation of caspase-9 by phosphorylation 23
3.4 CK2 inhibition switches ACD to apoptosis in HCN cells. 25
3.5 The cell death switching is dependent on ACD. 28
Ⅳ. Discussion 31
Ⅴ. Reference 34
Abstract in Korean 39
- URI
-
http://dgist.dcollection.net/common/orgView/200000281857
http://hdl.handle.net/20.500.11750/11915
- Degree
- Master
- Department
- Brain and Cognitive Sciences
- Publisher
- DGIST
- Related Researcher
-
-
Jeong, Youngtae
- Research Interests Stem cells; Cancer; Precision medicine; Regenerative medicine; Nrf2; antioxidant
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- Files in This Item:
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- Appears in Collections:
- Department of Brain Sciences Theses Master
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