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CK2-mediated phosphorylation of caspase-9 switches autophagic cell death to apoptosis in adult hippocampal neural stem cells following corticosterone treatment.

Title
CK2-mediated phosphorylation of caspase-9 switches autophagic cell death to apoptosis in adult hippocampal neural stem cells following corticosterone treatment.
Authors
Ye Won Lee
DGIST Authors
Lee, Ye Won; Jeong, YoungtaeYu, Seong-Woon
Advisor(s)
유성운
Co-Advisor(s)
Youngtae Jeong
Issue Date
2020
Available Date
2020-06-10
Degree Date
2020-02
Type
Thesis
Description
Autophagic cell death (ACD), Apoptosis, Corticosterone, Hippocampal neural stem (HCN) cells, Casein kinase 2 (CK 2), Caspase 9
Table Of Contents
Ⅰ. Introduction 1 Ⅱ. Material and Methods 11 2.1 Reagents and antibodies 11 2.2 Cell culture 11 2.3 Transfection, Expression vectors 12 2.4 Cell death assay 13 2.5 Western blotting 13 2.6 Caspase-9 activity assay 15 2.7 Immunocytochemistry 16 2.8 Live cell imaging 16 2.9 Statistical analysis 17 Ⅲ. Results 18 3.1 CASP9 is activated upon CORT-induced ACD in HCN cells. 18 3.2 Genetic ablation of CASP9 impairs CORT-induced ACD. 21 3.3 Regulation of caspase-9 by phosphorylation 23 3.4 CK2 inhibition switches ACD to apoptosis in HCN cells. 25 3.5 The cell death switching is dependent on ACD. 28 Ⅳ. Discussion 31 Ⅴ. Reference 34 Abstract in Korean 39
URI
http://dgist.dcollection.net/common/orgView/200000281857
http://hdl.handle.net/20.500.11750/11915
DOI
10.22677/Theses.200000286669
Degree
Master
Department
Brain and Cognitive Sciences
University
DGIST
Related Researcher
  • Author Yu, Seong-Woon Laboratory of Neuronal Cell Death
  • Research Interests Molecular mechanisms of neuronal cell death and neurodegeneration
Files:
There are no files associated with this item.
Collection:
Department of Brain and Cognitive SciencesThesesMaster


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