Abstract ……………………………………………………………………………………………………………………… i List of contents …………………………………………………………………………………………………………… iii List of tables ………………………………………………………………………………………………………………… v List of figures ………………………………………………………………………………………………………………… v
3. RESULTS …………………………………………………………………………………………………………………… 15 3-1. The HOOK region of β2 subunit regulates inactivation kinetics and PIP2 sensitivity of CaV2.2 channels ………………………………………………………………………………………………………… 15 3-2. Charged amino acids in the HOOK region mainly determine gating properties of CaV2.2 channels ………………………………………………………………………………………………………… 27 3-3. The short form of the HOOK region in β2c-short subunit shows similar regulatory effects on PIP2 sensitivity and inactivation of CaV2.2 channels ……………………………………………………………… 35 3-4. The B-domain of the HOOK region tethers β subunit to plasma membrane via electrostatic and hydrophobic interactions …………………………………………………………………………………………… 38 3-5. Distance between plasma membrane and CaV β subunit is critically important for modulating inactivation kinetics and PIP2 sensitivity of CaV2.2 channels …………………………………………………… 46 3-6. Conformational shift of I-II loop mainly determines gating properties of CaV2.2 channels ………… 53 3-7. Polybasic residues at the C-terminal end of I-II loop mainly determine PIP2 and muscarinic modulation of CaV2.2 channels ………………………………………………………………………………………… 66
Research Interests
Molecular mechanisms of epilepsy and sensory pain transmission; Signaling mechanism of ion channel regulation and membrane excitability; 분자전기생리; 간질 및 통증의 분자적 기전 연구