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Red blood cell study at different temperatures with holographic imaging informatics

Title
Red blood cell study at different temperatures with holographic imaging informatics
Authors
Minwoo Sim
DGIST Authors
Sim, Minwoo; Park, SanghyunMoon, Inkyu
Advisor(s)
문인규
Co-Advisor(s)
Sanghyun Park
Issue Date
2020
Available Date
2020-06-23
Degree Date
2020-02
Type
Thesis
Description
적혈구세포 형상분석, 세포막 변동률 분석, 디지털 홀로그래픽 현미경, 항상성
Abstract
The optimal functionality of red blood cells is closely associated with the surrounding environment. This study was undertaken to analyze the changes in membrane profile, mean hemoglobin content (MCH), and cell membrane fluctuations (CMF) of healthy red blood cells (RBC) at various temperatures. The temperature was elevated from 17℃ to 41℃ within a duration of less than one hour, and the holograms were recorded by a CCD camera in an off-axis digital holographic microscopy(DHM) system. After hologram reconstruction, we extracted single RBCs and evaluated their morphologically related features (projected surface area and sphericity coefficient), MCH, and CMF. We observed that elevating the temperature results in changes in the 3D profile. Since CMF amplitude is highly correlated to the bending curvature of RBC membrane, temperature-induced shape changes can alter CMF’s map and amplitudemainly larger fluctuations appear in dimple area at a higher temperature. Regardless of the shape changes, no alterations in MCH were seen with temperature variation.
Table Of Contents
Ⅰ. INTRODUCTION 1 1.1 Background 1 1.2 Label-free digital holographic imaging 3 Ⅱ. METHODS 7 2.1 Red blood cell sample preparations 7 Ⅲ. RESULTS AND DISCUSSIONS 8 3.1 Biochemical and morphological parameters 8 3.2 Discussions 10 3.2.1 RBC trapped between cover slip and glass 10 3.2.1 RBCs imaged on chamber 15 Ⅳ. CONCLUSIONS 17 Abbreviations 18 References 19
URI
http://dgist.dcollection.net/common/orgView/200000285143
http://hdl.handle.net/20.500.11750/11965
DOI
10.22677/Theses.200000285143
Degree
Master
Department
Robotics Engineering
University
DGIST
Related Researcher
  • Author Moon, Inkyu Intelligent Imaging and Vision Systems Laboratory
  • Research Interests
Files:
Collection:
Department of Robotics EngineeringThesesMaster


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