Cited 6 time in webofscience Cited 7 time in scopus

PTPσ Controls Presynaptic Organization of Neurotransmitter Release Machinery at Excitatory Synapses

Title
PTPσ Controls Presynaptic Organization of Neurotransmitter Release Machinery at Excitatory Synapses
Authors
Han, Kyung AhLee, Hee-YoonLim, DongseokShin, JungsuYoon, Taek HanLee, ChooungkuRhee, Jeong-SeopLiu, XinranUm, Ji WonChoi, Se-YoungKo, Jaewon
DGIST Authors
Um, Ji WonKo, Jaewon
Issue Date
2020-06
Citation
iScience, 23(6), 101203
Type
Article
Article Type
Article
Keywords
TYROSINE-PHOSPHATASE-SIGMALONG-TERM POTENTIATIONMICE LACKINGLAR-RPTPSRECEPTORPROTEINDELTAREGENERATIONCOMPLEXESNEUREXIN
ISSN
2589-0042
Abstract
Leukocyte common antigen-related receptor tyrosine phosphatases (LAR-RPTPs) are evolutionarily conserved presynaptic organizers. The synaptic role of vertebrate LAR-RPTPs in vivo, however, remains unclear. In the current study, we analyzed the synaptic role of PTPσ using newly generated, single conditional knockout (cKO) mice targeting PTPσ. We found that the number of synapses was reduced in PTPσ cKO cultured neurons in association with impaired excitatory synaptic transmission, abnormal vesicle localization, and abnormal synaptic ultrastructure. Strikingly, loss of presynaptic PTPσ reduced neurotransmitter release prominently at excitatory synapses, concomitant with drastic reductions in excitatory innervations onto postsynaptic target areas in vivo. Furthermore, loss of presynaptic PTPσ in hippocampal CA1 pyramidal neurons had no impact on postsynaptic glutamate receptor responses in subicular pyramidal neurons. Postsynaptic PTPσ deletion had no effect on excitatory synaptic strength. Taken together, these results demonstrate that PTPσ is a bona fide presynaptic adhesion molecule that controls neurotransmitter release and excitatory inputs. © 2020 The Author(s)
URI
http://hdl.handle.net/20.500.11750/12034
DOI
10.1016/j.isci.2020.101203
Publisher
Cell Press
Related Researcher
  • Author Ko, Jaewon Laboratory of Synapse Formation and Function
  • Research Interests Synapse Formation and Function; Neural Circuits; 뇌질환; animal model
Files:
Collection:
Department of Brain and Cognitive SciencesSynapse Disorder Laboratory1. Journal Articles
Department of Brain and Cognitive SciencesLaboratory of Synapse Formation and Function1. Journal Articles


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