Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Younghwan | ko |
dc.contributor.author | Lee, Ji-Won | ko |
dc.contributor.author | Nam, Hyeri | ko |
dc.contributor.author | Yu, Seong-Woon | ko |
dc.date.accessioned | 2020-08-19T10:46:29Z | - |
dc.date.available | 2020-08-19T10:46:29Z | - |
dc.date.created | 2020-07-02 | - |
dc.date.issued | 2020-06 | - |
dc.identifier.citation | Molecular Brain, v.13, no.1, pp.88 | - |
dc.identifier.issn | 1756-6606 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/12247 | - |
dc.description.abstract | Microglia are macrophages resident in the central nervous system. C-X3-C motif chemokine receptor 1 (CX3CR1) is a Gαi-coupled seven-transmembrane protein exclusively expressed in the mononuclear phagocyte system including microglia, as well as intestinal and kidney macrophages. Cx3cr1 CreERT2 mice express Cre recombinase in a tamoxifen-inducible manner and have been widely used to delete target genes in microglia, since microglia are long-lived cells and outlive peripheral macrophages, which continuously turn over and lose their gene modification over time. ATG7 is an E1-like enzyme that plays an essential role in two ubiquitin-like reactions, ATG12-ATG5 conjugation and LC3-lipidation in autophagy. To study the role of ATG7 in adult microglia, we generated Cx3cr1 CreERT2 :Atg7 fl/fl mice and deleted Atg7 at the age of 8 weeks, and found induction of intestinal adhesion. Since intestinal adhesion is caused by excessive inflammation, these results suggest that deletion of Atg7 in intestinal macrophages even for a short time results in inflammation that cannot be rescued by replenishment with wild-type intestinal macrophages. Our finding suggests that, depending on the roles of the gene, Cx3cr1-Cre-mediated gene deletion may yield unanticipated physiological outcomes outside the central nervous system, and careful necropsy is necessary to assure the microglia-specific roles of the target gene. © 2020 The Author(s). | - |
dc.language | English | - |
dc.publisher | BioMed Central | - |
dc.title | Cx3cr1(CreERT2)-driven Atg7 deletion in adult mice induces intestinal adhesion | - |
dc.type | Article | - |
dc.identifier.doi | 10.1186/s13041-020-00630-4 | - |
dc.identifier.wosid | 000540962900001 | - |
dc.identifier.scopusid | 2-s2.0-85086299202 | - |
dc.type.local | Article(Overseas) | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.citationVolume | 13 | - |
dc.identifier.citationNumber | 1 | - |
dc.identifier.citationStartPage | 88 | - |
dc.identifier.citationTitle | Molecular Brain | - |
dc.type.journalArticle | Article | - |
dc.description.isOpenAccess | Y | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | SUSCEPTIBILITY | - |
dc.subject.keywordPlus | MONOCYTES | - |
dc.subject.keywordPlus | REVEALS | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.contributor.affiliatedAuthor | Yu, Seong-Woon | - |