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Effects of Dimethyl Sulfoxide on the Pluripotency and Differentiation Capacity of Mouse Embryonic Stem Cells

Title
Effects of Dimethyl Sulfoxide on the Pluripotency and Differentiation Capacity of Mouse Embryonic Stem Cells
Authors
Yi, Jun-KooPark, SongHa, Jae-JungKim, Dae-HyunHuang, HaiPark, Si-JunLee, Mee-HyunRyoo, Zae-YoungKim, Sung-HyunKim, Myoung-Ok
DGIST Authors
Yi, Jun-Koo; Park, Song; Ha, Jae-Jung; Kim, Dae-Hyun; Huang, Hai; Park, Si-Jun; Lee, Mee-Hyun; Ryoo, Zae-Young; Kim, Sung-Hyun; Kim, Myoung-Ok
Issue Date
2020-09
Citation
Cellular Reprogramming, 22(5), 244-253
Type
Article
Article Type
Article
Author Keywords
mESCsDMSOLIFpluripotency markers
Keywords
DNA METHYLATIONIN-VITROSELF-RENEWALEXPRESSION5-HYDROXYMETHYLCYTOSINEMODELDMSOMAINTAINSDYNAMICSNETWORK
ISSN
2152-4971
Abstract
Mouse embryonic stem cells (mESCs) go through self-renewal in the existence of the cytokine leukemia inhibitory factor (LIF). LIF is added to the mouse stem cells culture medium, and its removal results in fast differentiation. Dimethyl sulfoxide (DMSO) is one of the most used solvents in drug test. We exposed 4-day mESC cultures to different concentrations of DMSO (0.1%, 0.5%, 1.0%, and 2.0%) to identify the safest dose exhibiting efficacy as a solvent. mESCs grown under general pluripotency conditions in the absence of LIF were treated with DMSO. In addition, as a control for differentiation, mESCs were grown in the absence of LIF. DMSO upregulated the mRNA expression level of pluripotency markers. Moreover, DMSO reduced the mRNA expression levels of ectodermal marker (beta-tubulin3), mesodermal marker (Hand1), and endodermal markers (Foxa2 and Sox17) in mESCs. These results indicate that DMSO treatment enhances the pluripotency and disrupts the differentiation of mESCs. We also show that members of the Tet oncogene family are critical to inhibiting the differentiation and methylation of mESCs. DMSO is appropriate to sustain the pluripotency of mESCs in the absence of LIF, and that mESCs can be sustained in an undifferentiated state using DMSO. Therefore, DMSO may, in part, function as a substitute for LIF.
URI
http://hdl.handle.net/20.500.11750/12412
DOI
10.1089/cell.2020.0006
Publisher
Mary Ann Liebert Inc.
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