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dc.contributor.author Kim, R. ko
dc.contributor.author Youn, Y.-S. ko
dc.contributor.author Kang, M. ko
dc.contributor.author Kim, Eunjoo ko
dc.date.accessioned 2021-01-22T06:46:49Z -
dc.date.available 2021-01-22T06:46:49Z -
dc.date.created 2020-12-03 -
dc.date.issued 2020-11 -
dc.identifier.citation Scientific Reports, v.10, no.1, pp.20461 -
dc.identifier.issn 2045-2322 -
dc.identifier.uri http://hdl.handle.net/20.500.11750/12593 -
dc.description.abstract Guanine-rich quadruplex (G-QD) are formed by conversion of nucleotides with specific sequences by stabilization of positively charged K+ or Na+. These G-QD structures differentially absorb two-directional (right- and left-handed) circularly polarized light, which can discriminate the parallel or anti-parallel structures of G-QDs. In this study, G-QDs stabilized by Pb2+ were analyzed by a circular dichroism (CD) spectroscopy to determine Pb2+ concentration in water samples. Thrombin aptamer (TBA), PS2.M, human telomeric DNA (HTG), AGRO 100, and telomeric related sequence (T2) were studied to verify their applicability as probes for platform- and label-free detection of Pb2+ in environmental as well as laboratory samples. Among these nucleotides, TBA and PS2.M exhibited higher binding constants for Pb2+, 1.20–2.04 × 106/M at and 4.58 × 104–1.09 × 105/M at 100 micromolar and 100 mM K+ concentration, respectively. They also exhibited excellent selectivity for Pb2+ than for Al3+, Cu2+, Ni2+, Fe3+, Co2+, and Cr2+. When Pb2+ was spiked into an effluent sample from a wastewater treatment plant (WWTP), its existence was detected by CD spectroscopy following a simple addition of TBA or PS2.M. By the addition of TBA and PS2.M, the Pb2+ signals were observed in effluent samples over 0.5 micromolar (100 ppb) concentration. Furthermore, PS2.M caused a Pb2+-specific absorption band in the effluent sample without spiking of Pb2+, and could be induced to G-QD structure by the background Pb2+ concentration in the effluent, 0.159 micromolar concentration (3.30 ppb). Taken together, we propose that TBA and PS2.M are applicable as platform- and label-free detection probes for monitoring Pb2+ in environmental samples such as discharged effluent from local WWTPs, using CD spectroscopy. © 2020, The Author(s). -
dc.language English -
dc.publisher Nature Research -
dc.title Platform- and label-free detection of lead ions in environmental and laboratory samples using G-quadraplex probes by circular dichroism spectroscopy -
dc.type Article -
dc.identifier.doi 10.1038/s41598-020-77449-5 -
dc.identifier.wosid 000596283100020 -
dc.identifier.scopusid 2-s2.0-85096612992 -
dc.type.local Article(Overseas) -
dc.type.rims ART -
dc.description.journalClass 1 -
dc.contributor.localauthor Kim, Eunjoo -
dc.contributor.nonIdAuthor Kim, R. -
dc.contributor.nonIdAuthor Youn, Y.-S. -
dc.contributor.nonIdAuthor Kang, M. -
dc.identifier.citationVolume 10 -
dc.identifier.citationNumber 1 -
dc.identifier.citationStartPage 20461 -
dc.identifier.citationTitle Scientific Reports -
dc.type.journalArticle Article -
dc.description.isOpenAccess Y -
dc.subject.keywordPlus DNA SECONDARY STRUCTURES -
dc.subject.keywordPlus G-QUADRUPLEX -
dc.subject.keywordPlus HUMAN TELOMERE -
dc.subject.keywordPlus STABILITY -
dc.subject.keywordPlus PB2+ -

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