Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Kim, Raeyeong | - |
dc.contributor.author | Youn, Young-Sang | - |
dc.contributor.author | Kang, Misook | - |
dc.contributor.author | Kim, Eunjoo | - |
dc.date.accessioned | 2021-01-22T06:46:49Z | - |
dc.date.available | 2021-01-22T06:46:49Z | - |
dc.date.created | 2020-12-03 | - |
dc.date.issued | 2020-12 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/12593 | - |
dc.description.abstract | Guanine-rich quadruplex (G-QD) are formed by conversion of nucleotides with specific sequences by stabilization of positively charged K+ or Na+. These G-QD structures differentially absorb two-directional (right- and left-handed) circularly polarized light, which can discriminate the parallel or anti-parallel structures of G-QDs. In this study, G-QDs stabilized by Pb2+ were analyzed by a circular dichroism (CD) spectroscopy to determine Pb2+ concentration in water samples. Thrombin aptamer (TBA), PS2.M, human telomeric DNA (HTG), AGRO 100, and telomeric related sequence (T2) were studied to verify their applicability as probes for platform- and label-free detection of Pb2+ in environmental as well as laboratory samples. Among these nucleotides, TBA and PS2.M exhibited higher binding constants for Pb2+, 1.20–2.04 × 106/M at and 4.58 × 104–1.09 × 105/M at 100 micromolar and 100mMK+ concentration, respectively. They also exhibited excellent selectivity for Pb2+ than for Al3+, Cu2+, Ni2+, Fe3+, Co2+, and Cr2+. When Pb2+ was spiked into an effluent sample from a wastewater treatment plant (WWTP), its existence was detected by CD spectroscopy following a simple addition of TBA or PS2.M. By the addition of TBA and PS2.M, the Pb2+ signals were observed in effluent samples over 0.5 micromolar (100ppb) concentration. Furthermore, PS2.M caused a Pb2+-specific absorption band in the effluent sample without spiking of Pb2+, and could be induced to G-QD structure by the background Pb2+ concentration in the effluent, 0.159 micromolar concentration (3.30ppb). Taken together, we propose that TBA and PS2.M are applicable as platform- and label-free detection probes for monitoring Pb2+ in environmental samples such as discharged effluent from local WWTPs, using CD spectroscopy. © 2020, The Author(s). | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.title | Platform- and label-free detection of lead ions in environmental and laboratory samples using G-quadraplex probes by circular dichroism spectroscopy | - |
dc.type | Article | - |
dc.identifier.doi | 10.1038/s41598-020-77449-5 | - |
dc.identifier.scopusid | 2-s2.0-85096612992 | - |
dc.identifier.bibliographicCitation | Scientific Reports, v.10, no.1, pp.20461 | - |
dc.description.isOpenAccess | TRUE | - |
dc.subject.keywordPlus | DNA SECONDARY STRUCTURES | - |
dc.subject.keywordPlus | G-QUADRUPLEX | - |
dc.subject.keywordPlus | HUMAN TELOMERE | - |
dc.subject.keywordPlus | STABILITY | - |
dc.subject.keywordPlus | PB2+ | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 20461 | - |
dc.citation.title | Scientific Reports | - |
dc.citation.volume | 10 | - |