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Oncostatin M enhances osteogenic differentiation of dental pulp stem cells derived from supernumerary teeth

Title
Oncostatin M enhances osteogenic differentiation of dental pulp stem cells derived from supernumerary teeth
Authors
Kim, YounghwanLee, JeongsangSeo, EunjinPark, JaekyungYea, KyungmooShin, JonghyunJang, IlhoJeong, Taesung
DGIST Authors
Kim, Younghwan; Lee, Jeongsang; Seo, Eunjin; Park, Jaekyung; Yea, Kyungmoo; Shin, Jonghyun; Jang, Ilho; Jeong, Taesung
Issue Date
2020-08
Citation
Biochemical and Biophysical Research Communications, 529(2), 169-174
Type
Article
Article Type
Article
Author Keywords
Dental pulp stem cellMesenchymal stem cellOncostatin MSupernumerary tooth
Keywords
CYTOKINEMOUSE
ISSN
0006-291X
Abstract
Supernumerary tooth (ST) may arise from uncertain developmental abnormalities or underlying genetic causes, and the extraction at the early age is recommended. Dental pulp stem cells (DPSCs) are the valuable resource for the regeneration of tooth and related craniofacial structures. DPSCs isolated from ST (sDPSCs) have not been fully characterized despite the potential in the applications. The objectives of this study are the efficient isolation of sDPSCs and the analysis of the properties as stem cells. sDPSCs were established by hammer-cracking and separation of the intact pulp from ST. sDPSCs in the culture were examined by light microscope and flow cytometer for the morphology and the surface marker expression. sDPSCs exhibited the cellular morphology of typical mesenchymal stem cells and expressed CD44, CD73, CD90, CD105 and CD166, but not CD14, CD34 or CD45. sDPSCs showed the differentiation potential toward osteogenic, chondrogenic and adipogenic lineages. During osteogenic differentiation, the stimulation by Oncostatin M enhanced the differentiation and significantly increased the expression of genes involved in the hard tissue repair, such as BMP2, BMP4, BMP6 and RUNX2. sDPSCs can be effectively derived from ST and displays the characteristics of mesenchymal stem cells in the maintenance and the differentiation. sDPSCs satisfies the quality as DPSCs thus provide the valuable resource to the regenerative therapy. © 2020 Elsevier Inc.
URI
http://hdl.handle.net/20.500.11750/12674
DOI
10.1016/j.bbrc.2020.06.013
Publisher
Elsevier B.V.
Related Researcher
  • Author Yea, Kyungmoo Protein Engineering Lab
  • Research Interests Antibody, Engineering, Phage Display, Therapeutics, Immune, Exosome, Translational, Cytokine
Files:
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Collection:
Department of New BiologyProtein Engineering Lab1. Journal Articles


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