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OsASN1 Overexpression in Rice Increases Grain Protein Content and Yield under Nitrogen-Limiting Conditions

Title
OsASN1 Overexpression in Rice Increases Grain Protein Content and Yield under Nitrogen-Limiting Conditions
Authors
Lee, SichulPark, JoonheumLee, JinwonShin, DongjinMarmagne, AnneLim, Pyung OkMasclaux-Daubresse, CelineAn, GynheungNam, Hong Gil
DGIST Authors
Lee, Sichul; Park, Joonheum; Lee, Jinwon; Shin, Dongjin; Marmagne, Anne; Lim, Pyung Ok; Masclaux-Daubresse, Celine; An, Gynheung; Nam, Hong Gil
Issue Date
2020-07
Citation
Plant and Cell Physiology, 61(7), 1309-1320
Type
Article
Article Type
Article
Author Keywords
Asparagine synthetase 1Grain quality and yieldKnockoutNitrogen use efficiencyOverexpressionRice
Keywords
CYTOSOLIC GLUTAMINE-SYNTHETASEASPARAGINE-SYNTHETASEUSE EFFICIENCYGENE-EXPRESSIONARABIDOPSISASSIMILATIONAMMONIUMMETABOLISMTRANSPORTERLESSONS
ISSN
0032-0781
Abstract
Nitrogen (N) is a major limiting factor affecting crop yield in unfertilized soil. Thus, cultivars with a high N use efficiency (NUE) and good grain protein content (GPC) are needed to fulfill the growing food demand and to reduce environmental burden. This is especially true for rice (Oryza sativa L.) that is cultivated with a high input of N fertilizer and is a primary staple food crop for more than half of the global population. Here, we report that rice asparagine synthetase 1 (OsASN1) is required for grain yield and grain protein contents under both N-sufficient (conventional paddy fields) and N-limiting conditions from analyses of knockout mutant plants. In addition, we show that overexpression (OX) of OsASN1 results in better nitrogen uptake and assimilation, and increased tolerance to N limitation at the seedling stage. Under field conditions, the OsASN1 OX rice plants produced grains with increased N and protein contents without yield reduction compared to wild-type (WT) rice. Under N-limited conditions, the OX plants displayed increased grain yield and protein content with enhanced photosynthetic activity compared to WT rice. Thus, OsASN1 can be an effective target gene for the development of rice cultivars with higher grain protein content, NUE, and grain yield under N-limiting conditions. © The Author(s) 2020. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists.
URI
http://hdl.handle.net/20.500.11750/12675
DOI
10.1093/pcp/pcaa060
Publisher
Oxford University Press
Related Researcher
  • Author Lim, Pyung Ok Plant Senescence Laboratory
  • Research Interests Aging and programmen cell death in plants; RNA-based regulation of leaf aging; Synthetic genetics-based aging reprogramming
Files:
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Collection:
Department of New BiologyPlant Senescence Laboratory1. Journal Articles
Department of New BiologyCBRG(Complex Biology Research Group)1. Journal Articles


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