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Lin28a attenuates TGF-beta-induced renal fibrosis

Title
Lin28a attenuates TGF-beta-induced renal fibrosis
Authors
Hwang, Yeo JinGwon-Soo JungJun-Hyuk ChoiLee, Kyeong-Min
DGIST Authors
Hwang, Yeo Jin; Gwon-Soo Jung; Jun-Hyuk Choi; Lee, Kyeong-Min
Issue Date
2020-11
Citation
BMB Reports, 53(11), 594-599
Type
Article
Article Type
Article
Author Keywords
Lin28aRenal fibrosisRenal tubular epithelial cellSMAD3TGF-beta signaling
Keywords
GROWTH-FACTOR-BETAEXTRACELLULAR-MATRIXOBSTRUCTIVE NEPHROPATHYMESSENGER-RNAEPITHELIAL-CELLSEXPRESSIONPATHWAYLET-7PROLIFERATIONACTIVATION
ISSN
1976-6696
Abstract
Lin28a has diverse functions including regulation of cancer, reprogramming and regeneration, but whether it promotes injury or is a protective reaction to renal injury is unknown. We studied how Lin28a acts in unilateral ureteral obstruction (UUO)-induced renal fibrosis following unilateral ureteral obstruction, in a mouse model. We further defined the role of Lin28a in transforming growth factor (TGF)-signaling pathways in renal fibrosis through in vitro study using human tubular epithelium-like HK-2 cells. In the mouse unilateral ureteral obstruction model, obstruction markedly decreased the expression of Lin28a, increased the expression of renal fibrotic markers such as type I collagen, α-SMA, vimentin and fibronectin. In TGF-β-stimulated HK-2 cells, the expression of Lin28a was reduced and the expression of renal fibrotic markers such as type I collagen, α-SMA, vimentin and fibronectin was increased. Adenovirus-mediated overexpression of Lin28a inhibited the expression of TGF-?-stimulated type I collagen, α-SMA, vimentin and fibronectin. Lin28a inhibited TGF-β-stimulated SMAD3 activity, via inhibition of SMAD3 phosphorylation, but not the MAPK pathway ERK, JNK or p38. Lin28a attenuates renal fibrosis in obstructive nephropathy, making its mechanism a possible therapeutic target for chronic kidney disease. [BMB Reports 2020; 53(11): 594-599] © 2020 by the The Korean Society for Biochemistry and Molecular Biology
URI
http://hdl.handle.net/20.500.11750/12772
DOI
10.5483/BMBRep.2020.53.11.153
Publisher
생화학분자생물학회
Related Researcher
Files:
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Collection:
Division of Biotechnology1. Journal Articles


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