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Mass spectrometry imaging of untreated wet cell membranes in solution using single-layer graphene

Title
Mass spectrometry imaging of untreated wet cell membranes in solution using single-layer graphene
Authors
Lim, HeejinLee, Sun YoungPark, YeruemJin, HyeonggyuSeo, DaehaJang, Yun HeeMoon, DaeWon
DGIST Authors
Lim, Heejin; Lee, Sun Young; Park, Yeruem; Jin, Hyeonggyu; Seo, DaehaJang, Yun Hee; Moon, DaeWon
Issue Date
2021-03
Citation
Nature Methods, 18(3), 316-320
Type
Article
Article Type
Article; Early Access
ISSN
1548-7091
Abstract
We report a means by which atomic and molecular secondary ions, including cholesterol and fatty acids, can be sputtered through single-layer graphene to enable secondary ion mass spectrometry (SIMS) imaging of untreated wet cell membranes in solution at subcellular spatial resolution. We can observe the intrinsic molecular distribution of lipids, such as cholesterol, phosphoethanolamine and various fatty acids, in untreated wet cell membranes without any labeling. We show that graphene-covered cells prepared on a wet substrate with a cell culture medium reservoir are alive and that their cellular membranes do not disintegrate during SIMS imaging in an ultra-high-vacuum environment. Ab initio molecular dynamics calculations and ion dose-dependence studies suggest that sputtering through single-layer graphene occurs through a transient hole generated in the graphene layer. Cholesterol imaging shows that methyl-β-cyclodextrin preferentially extracts cholesterol molecules from the cholesterol-enriched regions in cell membranes. © 2021, The Author(s), under exclusive licence to Springer Nature America, Inc.
URI
http://hdl.handle.net/20.500.11750/12982
DOI
10.1038/s41592-020-01055-6
Publisher
Nature Publishing Group
Related Researcher
  • Author Jang, Yun Hee CMMM Lab(Curious Minds Molecular Modeling Laboratory)
  • Research Interests Multiscale molecular modeling (quantum mechanics calculation; molecular dynamics simulation) : Supercomputer-assisted molecular-level understanding of materials and their chemistry; which leads to rational design of high-performance organic-inorganic-hybrid materials for clean and renewable energy as well as low-energy-consumption electronic devices
Files:
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Collection:
Department of New BiologyETC1. Journal Articles
Department of Physics and ChemistrySMALL LAB(Single Molecule Approaches to ceLL Lab)1. Journal Articles
Department of Energy Science and EngineeringCMMM Lab(Curious Minds Molecular Modeling Laboratory)1. Journal Articles


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