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RGD-containing elastin-like polypeptide induces the β-cell adhesion and proliferation and improves islet transplantation outcomes in diabetic mice

Title
RGD-containing elastin-like polypeptide induces the β-cell adhesion and proliferation and improves islet transplantation outcomes in diabetic mice
Authors
Lee, Kyeong-MinHwang, Yeo-JinChoi, Jun-Hyeok
DGIST Authors
Lee, Kyeong-Min; Hwang, Yeo-Jin
Issue Date
2019-11-01
Citation
2019 한국공업화학회 추계 총회 및 학술대회
Type
Conference
Abstract
Proliferation of beta cell plays important role in the maintenance of beta cell mass and function. Extracellular matrix (ECM) components increase the adhesion and proliferation of pancreatic β- cells. In this study, we investigated whether RGD-modified elastin-like polypeptide (REP), as a bioactive matrix, could enhance the proliferation of β-cells and protect islets from cell death. REP effectively increased the cell adhesion and proliferation of β- cell. REP increased cyclin D1and D2 levels, and decreased p27 levels. REP controlled the gene expression of insulin, PDX-1 and BETA2. When beta cell cultured on REP, the pathway of Akt and Erk were stimulated. Co-transplantation of REP-treated islets and REP beneath the renal sub-capsule in streptozoticin-induced diabetic mice restored normoglycemia. Moreover, co-transplantation sites exhibited enhanced β-cell proliferation and vascularization. Thus, the REP treatment improve the proliferation, survival, and function of β-cell, and therapeutic potential of transplanted islets.
URI
http://hdl.handle.net/20.500.11750/14200
Publisher
한국공업화학회
Related Researcher
Files:
There are no files associated with this item.
Collection:
Division of Biotechnology2. Conference Papers


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