Cited 0 time in webofscience Cited 0 time in scopus

Nuclear TDP-43 regulates Golgi outposts in Drosophila neurons

Nuclear TDP-43 regulates Golgi outposts in Drosophila neurons
Lee, DavinPark, SungSoonChung, Chang GeonLee, Sung-Bae
DGIST Authors
Lee, Sung-Bae
Issue Date
8th BCS student symposium
TDP-43 is a heterogeneous nuclear ribonucleoprotein (HnRNP) whose abnormal clearance in the nucleus and aberrant accumulation in the cytoplasm is frequently observed in several neurodegenerative diseases such as ALS/FTD. Although molecular functions of TDP-43, such as mRNA processing, have been well characterized, its physiological role in neurons remains unclear. In this study, we elucidated the novel role of dTDP-43 in the formation of Golgi outposts (GOPs) in dendrites. We found that the nuclear localization of dTDP-43 determines the number of GOPs in Drosophila neurons. Loss-of-function genetic screening of previously characterized co-factors of dTDP-43 nuclear function showed that two pre-mRNA splicing partners, Hrb27C and Hrb98DE, are associated with dTDP-43-induced increase of GOPs. Additional loss-of-function genetic screening of NGS-derived differentially expressed genes (DEGs) in dTDP-43 overexpressed brain further identified several specific Rab GTPases and their interactors as effectors of this novel role of dTDP-43. Finally, we found that late endo-lysosomal compartments and plasma membrane supply were also increased following increased GOPs in dendrites of dTDP-43-expressing neurons. Taken together, our results suggest that TDP-43 contributes to the neuronal local membrane trafficking through the regulation of GOPs.
DGIST dept. of Brain & Cognitive Sciences
Related Researcher
  • Author Lee, Sung Bae Laboratory of Neurodegenerative Diseases and Aging
  • Research Interests Cellular mechanism of neurodegenerative diseases; Neuronal maintenance and remodeling; 퇴행성 뇌질환의 세포기전; 신경계 유지 및 리모델링 연구
There are no files associated with this item.
Department of Brain SciencesLaboratory of Neurodegenerative Diseases and Aging2. Conference Papers

qrcode mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.