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GC-QqQ-MS/MS analysis of free fatty acids changed by palmitate-induced lipophagy

GC-QqQ-MS/MS analysis of free fatty acids changed by palmitate-induced lipophagy
Park, SeokjaeOh, Tae SeokKim, Eun-Kyoung
DGIST Authors
Kim, Eun-Kyoung
Issue Date
2019 한국대사체학회 정기학술대회 및 제7차 정기총회
Current studies suggest that modification of hypothalamic fatty acid metabolism can improve energy homeostasis and prevent hyperphagia in obesity. Autophagy can provide energetically more efficient essential components such as free fatty acids (FFAs) by breaking down intracellular organelles. Among the types of selective autophagy, lipophagy is known to degrade cellular lipid droplets (LDs) and liberate FFAs which serve as an important pathway for lipid metabolism. Emerging evidence suggests that intracellular FFAs can translocate to the nucleus by lipid binding protein (LBP) and regulate transcription. We here report the metabolomic analysis of FFAs changed by palmitate-induced lipophagy in hypothalamic neurons using gas chromatography triple-quadrupole mass spectrometry (GC-QqQ-MS/MS). Our results show that palmitate induces lipophagy which in turn, significantly increases monounsaturated fatty acids (MUFAs) in nuclear fractionation. The effect of palmitate-induced lipophagy in increasing MUFAs is blunted in autophagy-related gene 5 (ATG5) knockout cells. In addition, the lipophagy-induced increase of palmitoleic acid (C16:1) is also blunted in LBP knockdown cells. These findings may broaden the understanding of hypothalamic lipophagy that regulates energy balance via changes in intracellular lipid metabolism. Furthermore, it is going to be a stepping stone for the development of therapeutic strategies for metabolic diseases.
Korea Metabolomics Society
Related Researcher
  • Author Kim, Eun-Kyoung Lab of Neuro-Metabolism & Neurometabolomic Research Center
  • Research Interests Neural functions in metabolic diseases; 뇌신경세포와 비만; 당뇨 등의 대사 질환 관련 연구
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Department of Brain SciencesLab of Neuro-Metabolism & Neurometabolomic Research Center2. Conference Papers

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