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Observation on caudal fin regeneration of zebrafish using high-spatial resolution mass spectrometry imaging (MSI) methods

Title
Observation on caudal fin regeneration of zebrafish using high-spatial resolution mass spectrometry imaging (MSI) methods
Authors
Kim, Jae YoungLee, Seon YoungMoon, DaeWon
DGIST Authors
Kim, Jae Young
Issue Date
2019-02-19
Citation
2019년 한국진공학회 겨울정기학술대회
Type
Conference
Abstract
We observed the molecular distribution changes that occurred during the regeneration of fresh zebrafish caudal fins using the recently developed ambient high-resolution mass spectrometry (MS) imaging technique of atmospheric pressure-nanoparticle and plasma assisted laser desorption ionization (AP-nanoPALDI) and time-of-flight secondary ion mass spectrometry (ToF-SIMS). The zebrafish caudal fin is not only a very appropriate bio-specimen for mass spectrometry due to its thickness of less than 200 micrometers but also a very important organ for tissue regeneration studies. Thus, mass spectrometry methods could be widely applied to the wound healing studies using caudal fins of zebrafish due to providing plenty of biomolecular information. The chemical mapping images obtained by two different MS methods seem to represent different spatial distributions of small molecules due to differences in detection/ionization methods, analytical procedures, and experimental conditions. However, careful observation and interpretation confirms that both MS imaging techniques provide important information about the regeneration of the zebrafish caudal fin. In addition, the complementary information of the bulk and surface sensitive analyses can be used to observe the regeneration phenomenon of the truncated caudal fin in more detail.
URI
http://hdl.handle.net/20.500.11750/14534
Publisher
한국진공학회
Files:
There are no files associated with this item.
Collection:
Department of Robotics and Mechatronics EngineeringRobotics Engineering Research Center2. Conference Papers


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