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Lin28a ameliorates glucotoxicity-induced beta-cell dysfunction and apoptosis

Title
Lin28a ameliorates glucotoxicity-induced beta-cell dysfunction and apoptosis
Authors
Hwang, Yeo JinJung, Gwon-SooJeon, WonbaeLee, Kyeong-Min
DGIST Authors
Hwang, Yeo Jin; Jung, Gwon-Soo; Jeon, WonbaeLee, Kyeong-Min
Issue Date
2021-04
Citation
BMB Reports, 54(4), 215-220
Type
Article
Author Keywords
ApoptosisGlucotoxicityInsulinLin28amTORPI3K/Akt
Keywords
INSULIN-RESISTANCEPANCREATIC-ISLETSCHRONIC EXPOSUREEXPRESSIONRATFAMILYBCL-2
ISSN
1976-6696
Abstract
An excessive and prolonged increase in glucose levels causes beta-cell dysregulation, which is accompanied by impaired insulin synthesis and secretion, a condition known as glucotoxicity. Although it is known that both Lin28a and Lin28b regulate glucose metabolism, other molecular mechanisms that may protect against glucotoxicity are poorly understood. We investigated whether Lin28a overexpression can improve glucotoxicity-induced beta-cell dysregulation in INS-1 and primary rat islet cells. INS-1, a rat insulinoma cell line was cultured and primary rat islet cells were isolated from SD-rats. To define the effect of Lin28a in chronic high glucose-induced beta-cell dysregulation, we performed several in vitro and ex-vivo experiments. Chronic exposure to high glucose led to a downregulation of Lin28a mRNA and protein expression, followed by a decrease in insulin mRNA expression and secretion in beta-cells. The mRNA and protein expression levels of PDX-1 and BETA2, were reduced; The levels of apoptotic factors, including c-caspase3 and the Bax/Bcl-2 ratio, were increased due to glucotoxicity. Adenovirus-mediated Lin28a overexpression in beta-cells reversed the glucotoxicity-induced reduction of insulin secretion and insulin mRNA expression via regulation of beta-cell-enriched transcription factors such as PDX-1 and BETA2. Adenovirus-mediated overexpression of Lin28a downregulated the glucotoxicity-induced upregulation of c-caspase3 levels and the Bax/Bcl-2 ratio, while inhibition of endogenous Lin28a by small interfering RNA resulted in their up-regulation. Lin28a counteracted glucotoxicity-induced downregulation of p-Akt and p-mTOR. Our results suggest that Lin28a protects pancreatic beta-cells from glucotoxicity through inhibition of apoptotic factors via the PI3 kinase/Akt/mTOR pathway. © 2021 by the The Korean Society for Biochemistry and Molecular Biology
URI
http://hdl.handle.net/20.500.11750/15456
DOI
10.5483/BMBRep.2021.54.4.255
Publisher
The Biochemical Society of the Republic of Korea
Related Researcher
Files:
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Collection:
Division of Biotechnology1. Journal Articles


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