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Rapid and simple single-chamber nucleic acid detection system prepared through nature-inspired surface engineering

Title
Rapid and simple single-chamber nucleic acid detection system prepared through nature-inspired surface engineering
Authors
Park, JihyoWoo, SangwonKim, JiyeonLee, HakhoYoo, Yeong-EunHong, Seonki
DGIST Authors
Park, Jihyo; Woo, Sangwon; Kim, Jiyeon; Lee, Hakho; Yoo, Yeong-Eun; Hong, Seonki
Issue Date
2021-05
Citation
Theranostics, 11(14), 6735-6745
Type
Article
Author Keywords
colorimetric assayinjection moldingnucleic acid-based diagnosticspathogen detectionsurface coating
Keywords
silicon dioxideArticlecoating (procedure)colorimetrycontrolled studycost effectiveness analysisdiffusiongene amplificationgenetic engineeringgenetic proceduresin situ polymerase chain reactionincubation timemicrofluidicsmolecular diagnosismolecular pathologynucleic acid analysispHplasticityproof of conceptsurface propertytime factor
ISSN
1838-7640
Abstract
Background: Nucleic acid (NA)-based diagnostics enable a rapid response to various diseases, but current techniques often require multiple labor-intensive steps, which is a major obstacle to successful translation to a clinical setting. Methods: We report on a surface-engineered single-chamber device for NA extraction and in situ amplification without sample transfer. Our system has two reaction sites: A NA extraction chamber whose surface is patterned with micropillars and a reaction chamber filled with reagents for in situ polymerase-based NA amplification. These two sites are integrated in a single microfluidic device; we applied plastic injection molding for cost-effective, mass-production of the designed device. The micropillars were chemically activated via a nature-inspired silica coating to possess a specific affinity to NA. Results: As a proof-of-concept, a colorimetric pH indicator was coupled to the on-chip analysis of NA for the rapid and convenient detection of pathogens. The NA enrichment efficiency was dependent on the lysate incubation time, as diffusion controls the NA contact with the engineered surface. We could detect down to 1×103 CFU by the naked eye within one hour of the total assay time. Conclusion: We anticipate that the surface engineering technique for NA enrichment could be easily integrated as a part of various types of microfluidic chips for rapid and convenient nucleic acid-based diagnostics. © 2021 Ivyspring International Publisher. All rights reserved.
URI
http://hdl.handle.net/20.500.11750/15493
DOI
10.7150/thno.57153
Publisher
Ivyspring International Publisher
Related Researcher
  • Author Hong, Seonki Biomaterials & Biointerface Engineering Laboratory
  • Research Interests Bio-inspired organic materials; Polymeric biomaterials; Surface biofunctionalization; biochip fabrication
Files:
There are no files associated with this item.
Collection:
Department of Emerging Materials ScienceBiomaterials & Biointerface Engineering Laboratory1. Journal Articles


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