Cited time in webofscience Cited time in scopus

Rapid and simple single-chamber nucleic acid detection system prepared through nature-inspired surface engineering

Rapid and simple single-chamber nucleic acid detection system prepared through nature-inspired surface engineering
Park, JihyoWoo, SangwonKim, JiyeonLee, HakhoYoo, Yeong-EunHong, Seonki
DGIST Authors
Park, JihyoWoo, SangwonKim, JiyeonLee, HakhoYoo, Yeong-EunHong, Seonki
Issued Date
Author Keywords
colorimetric assayinjection moldingnucleic acid-based diagnosticspathogen detectionsurface coating
silicon dioxideArticlecoating (procedure)colorimetrycontrolled studycost effectiveness analysisdiffusiongene amplificationgenetic engineeringgenetic proceduresin situ polymerase chain reactionincubation timemicrofluidicsmolecular diagnosismolecular pathologynucleic acid analysispHplasticityproof of conceptsurface propertytime factor
Background: Nucleic acid (NA)-based diagnostics enable a rapid response to various diseases, but current techniques often require multiple labor-intensive steps, which is a major obstacle to successful translation to a clinical setting. Methods: We report on a surface-engineered single-chamber device for NA extraction and in situ amplification without sample transfer. Our system has two reaction sites: A NA extraction chamber whose surface is patterned with micropillars and a reaction chamber filled with reagents for in situ polymerase-based NA amplification. These two sites are integrated in a single microfluidic device; we applied plastic injection molding for cost-effective, mass-production of the designed device. The micropillars were chemically activated via a nature-inspired silica coating to possess a specific affinity to NA. Results: As a proof-of-concept, a colorimetric pH indicator was coupled to the on-chip analysis of NA for the rapid and convenient detection of pathogens. The NA enrichment efficiency was dependent on the lysate incubation time, as diffusion controls the NA contact with the engineered surface. We could detect down to 1×103 CFU by the naked eye within one hour of the total assay time. Conclusion: We anticipate that the surface engineering technique for NA enrichment could be easily integrated as a part of various types of microfluidic chips for rapid and convenient nucleic acid-based diagnostics. © 2021 Ivyspring International Publisher. All rights reserved.
Ivyspring International Publisher
Related Researcher
  • 홍선기 Hong, Seonki 화학물리학과
  • Research Interests Bio-inspired organic materials; Polymeric biomaterials; Surface biofunctionalization; biochip fabrication
Files in This Item:
2021_Theranostics_Jihyo_RNA device.pdf

2021_Theranostics_Jihyo_RNA device.pdf

기타 데이터 / 1.78 MB / Adobe PDF download
Appears in Collections:
Department of Physics and Chemistry Bioinspired Organic Materials Laboratory 1. Journal Articles


  • twitter
  • facebook
  • mendeley

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.