Cited time in webofscience Cited time in scopus

Magnetism-controlled assembly of composite stem cell spheroids for the biofabrication of contraction-modulatory 3D tissue

Title
Magnetism-controlled assembly of composite stem cell spheroids for the biofabrication of contraction-modulatory 3D tissue
Author(s)
Byun, HayeonLee, SangminJang, Gyu NamLee, HyoryongPark, SukhoShin, Heungsoo
DGIST Authors
Byun, HayeonLee, SangminJang, Gyu NamLee, HyoryongPark, SukhoShin, Heungsoo
Issued Date
2022-01
Type
Article
Author Keywords
bottom-up tissue engineeringmagnetic nanoparticlesmagnetic assemblystem cell spheroids
Keywords
IRON-OXIDE NANOPARTICLESSURFACE-CHARGEADHESIONCULTUREDIFFERENTIATIONPROLIFERATIONCYTOTOXICITYMANIPULATIONSCAFFOLDSGELATIN
ISSN
1758-5082
Abstract
Biofabrication of organ-like engineered 3D tissue through the assembly of magnetized 3D multi-cellular spheroids has been recently investigated in tissue engineering. However, the cytotoxicity of magnetic nanoparticles (MNPs) and contraction-induced structural deformation of the constructs have been major limitations. In this study, we developed a method to fabricate composite stem cell spheroids using MNP-coated fibers, alleviating MNP-mediated toxicity and controlling structural assembly under external magnetic stimuli. The MNP-coated synthetic fibers (MSFs) were prepared by coating various amounts of MNPs on the fibers via electrostatic interactions. The MSFs showed magnetic hysteresis and no cytotoxicity on 2D-cultured adipose-derived stem cells (ADSCs). The composite spheroids containing MSFs and ADSCs were rapidly formed in which the amount of impregnated MSFs modulated the spheroid size. The fusion of in vitro composite spheroids was then monitored at the contacting interface; the fused spheroids with over 10 mu g of MSF showed minimal contraction after 7 d, retaining around 90% of total area ratio regardless of the number of cells, indicating that the presence of fibers within the composite spheroid supported its structural maintenance. The fusion of MSF spheroids was modulated by external magnetic stimulation, and the effect of magnetic force on the movement and fusion of the spheroids was investigated using COMSOL simulation. Finally, ring and lamellar structures were successfully assembled using remote-controlled MSF spheroids, showing limited deformation and high viability up to 50 d during in vitro culture. In addition, the MSFs demonstrated no adverse effects on ADSC osteochondral differentiation. Altogether, we envision that our magnetic assembly system would be a promising method for the tissue engineering of structurally controlled organ-like constructs.
URI
http://hdl.handle.net/20.500.11750/15890
DOI
10.1088/1758-5090/ac318b
Publisher
IOS Press
Related Researcher
  • 박석호 Park, Sukho 로봇및기계전자공학과
  • Research Interests Biomedical Micro/Nano Robotics; Biomedical Devices and Instruments
Files in This Item:

There are no files associated with this item.

Appears in Collections:
Department of Robotics and Mechatronics Engineering Multiscale Biomedical Robotics Laboratory 1. Journal Articles

qrcode

  • twitter
  • facebook
  • mendeley

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE