Title

Knockdown of Primary Ciliary Component Gene in Hypothalamic Paraventricular Nucleus Oxytocin Neurons Impairs Social Behavior

DGIST Authors

Seree Park ; Han Kyoung Choe ; Jeongyeon Kim

Advisor

최한경

Co-Advisor(s)

Jeongyeon Kim

Issued Date

2022

Awarded Date

2022/02

Citation

Seree Park. (2022). Knockdown of Primary Ciliary Component Gene in Hypothalamic Paraventricular Nucleus Oxytocin Neurons Impairs Social Behavior. doi: 10.22677/thesis.200000593842

Type

Thesis

Subject

Oxytocin, Primary cilia, Ift88, Social recognition, Fiber photometry

Description

Oxytocin, Primary cilia, Ift88, Social recognition, Fiber photometry

Table Of Contents

I. Introduction 1
II. Materials and Methods 3
III. Results 9
3.1 Knockdown of Ift88 in PVN-Oxt neurons with shRNA reduced cilia length 9
3.2 Adult-onset inhibition of ciliogenesis with shIft88 in PVN-Oxt neurons selectively disrupted social recognition and memory 10
3.3 Ift88 knockdown in adult PVN-Oxt neurons did not change metabolic phenotype 11
3.4 Postnatal Ift88 knockdown in PVN-Oxt neurons did not alter axonal projection 12
3.5 Ciliary dysgenesis in adult PVN-Oxt neurons increased spontaneous EPSCs 13
3.6 PVN-Oxt neurons are activated by social stimuli, especially on social novelty 14
3.7 Cilia mutant PVN-Oxt neurons showed different neuronal response 16
IV. Discussion 37
V. References 41

URI

http://dgist.dcollection.net/common/orgView/200000593842
http://hdl.handle.net/20.500.11750/16298

DOI

10.22677/thesis.200000593842

Degree

Master

Department

Brain and Cognitive Sciences

Publisher

DGIST

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