Title

Role of CK2 in autophagic cell death following corticosterone treatment in adult hippocampal neural stem cells

Alternative Title

성체 해마신경줄기세포의 코르티코스테론 유도 오토파지 세포사멸에서의 CK2의 역할

DGIST Authors

Youngchae Lee ; Seong-Woon Yu ; Byung-Hoon Lee

Advisor

유성운

Co-Advisor(s)

Byung-Hoon Lee

Issued Date

2022

Awarded Date

2022/02

Citation

Youngchae Lee. (2022). Role of CK2 in autophagic cell death following corticosterone treatment in adult hippocampal neural stem cells. doi: 10.22677/thesis.200000593750

Type

Thesis

Subject

Autophagic cell death (ACD), Apoptosis, Corticosterone (CORT), Hippocampal neural stem (HCN) cells, Casein kinase 2 (CK2), Psychological stress.

Description

Autophagic cell death (ACD), Apoptosis, Corticosterone (CORT), Hippocampal neural stem (HCN) cells, Casein kinase 2 (CK2), Psychological stress.

Table Of Contents

Ⅰ. Introduction 1
Ⅱ. Material and Methods 3
2.1 Reagents and antibodies 3
2.2 Animals 3
2.3 Cell culture 4
2.4 Transfection 4
2.5 Immunocytochemistry 4
2.6 Cell death assay 5
2.7 Generation of stable knockdown HCN cells 5
2.8 Western blotting analysis 5
2.9 Statistical analysis 6
Ⅲ. Results 8
3.1 CORT induces ACD, not apoptosis in HCN cells 8
3.2 CK2 inhibition further increases CORT-induced death in HCN cells 12
3.3 Knockdown of CK2α subunit switches ACD to apoptosis 14
3.4 Knockdown of CK2β subunit switches ACD to apoptosis 17
3.5 Generation of NSC-specific, inducible CK2β cKO mice 20
Ⅳ. Discussion 24
Ⅴ. Reference 26
Abstract in Korean 30

URI

http://dgist.dcollection.net/common/orgView/200000593750
http://hdl.handle.net/20.500.11750/16319

DOI

10.22677/thesis.200000593750

Degree

Master

Department

Brain and Cognitive Sciences

Publisher

DGIST

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