Role of CK2 in autophagic cell death following corticosterone treatment in adult hippocampal neural stem cells
- Title
- Role of CK2 in autophagic cell death following corticosterone treatment in adult hippocampal neural stem cells
- Alternative Title
- 성체 해마신경줄기세포의 코르티코스테론 유도 오토파지 세포사멸에서의 CK2의 역할
- Author(s)
- Youngchae Lee
- DGIST Authors
- Youngchae Lee ; Seong-Woon Yu ; Byung-Hoon Lee
- Advisor
- 유성운
- Co-Advisor(s)
- Byung-Hoon Lee
- Issued Date
- 2022
- Awarded Date
- 2022/02
- Type
- Thesis
- Description
- Autophagic cell death (ACD), Apoptosis, Corticosterone (CORT), Hippocampal neural stem (HCN) cells, Casein kinase 2 (CK2), Psychological stress.
- Table Of Contents
-
Ⅰ. Introduction 1
Ⅱ. Material and Methods 3
2.1 Reagents and antibodies 3
2.2 Animals 3
2.3 Cell culture 4
2.4 Transfection 4
2.5 Immunocytochemistry 4
2.6 Cell death assay 5
2.7 Generation of stable knockdown HCN cells 5
2.8 Western blotting analysis 5
2.9 Statistical analysis 6
Ⅲ. Results 8
3.1 CORT induces ACD, not apoptosis in HCN cells 8
3.2 CK2 inhibition further increases CORT-induced death in HCN cells 12
3.3 Knockdown of CK2α subunit switches ACD to apoptosis 14
3.4 Knockdown of CK2β subunit switches ACD to apoptosis 17
3.5 Generation of NSC-specific, inducible CK2β cKO mice 20
Ⅳ. Discussion 24
Ⅴ. Reference 26
Abstract in Korean 30
- URI
-
http://dgist.dcollection.net/common/orgView/200000593750
http://hdl.handle.net/20.500.11750/16319
- Degree
- Master
- Department
- Brain and Cognitive Sciences
- Publisher
- DGIST
- Related Researcher
-
-
Yu, Seong-Woon
- Research Interests Molecular mechanisms of neuronal cell death and neurodegeneration
-
- Files in This Item:
-
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- Appears in Collections:
- Department of Brain Sciences Theses Master
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