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Role of CK2 in autophagic cell death following corticosterone treatment in adult hippocampal neural stem cells

Title
Role of CK2 in autophagic cell death following corticosterone treatment in adult hippocampal neural stem cells
Alternative Title
성체 해마신경줄기세포의 코르티코스테론 유도 오토파지 세포사멸에서의 CK2의 역할
Author(s)
Youngchae Lee
DGIST Authors
Youngchae LeeSeong-Woon YuByung-Hoon Lee
Advisor
유성운
Co-Advisor(s)
Byung-Hoon Lee
Issued Date
2022
Awarded Date
2022/02
Type
Thesis
Subject
Autophagic cell death (ACD), Apoptosis, Corticosterone (CORT), Hippocampal neural stem (HCN) cells, Casein kinase 2 (CK2), Psychological stress.
Description
Autophagic cell death (ACD), Apoptosis, Corticosterone (CORT), Hippocampal neural stem (HCN) cells, Casein kinase 2 (CK2), Psychological stress.
Table Of Contents
Ⅰ. Introduction 1
Ⅱ. Material and Methods 3
2.1 Reagents and antibodies 3
2.2 Animals 3
2.3 Cell culture 4
2.4 Transfection 4
2.5 Immunocytochemistry 4
2.6 Cell death assay 5
2.7 Generation of stable knockdown HCN cells 5
2.8 Western blotting analysis 5
2.9 Statistical analysis 6
Ⅲ. Results 8
3.1 CORT induces ACD, not apoptosis in HCN cells 8
3.2 CK2 inhibition further increases CORT-induced death in HCN cells 12
3.3 Knockdown of CK2α subunit switches ACD to apoptosis 14
3.4 Knockdown of CK2β subunit switches ACD to apoptosis 17
3.5 Generation of NSC-specific, inducible CK2β cKO mice 20
Ⅳ. Discussion 24
Ⅴ. Reference 26
Abstract in Korean 30
URI
http://dgist.dcollection.net/common/orgView/200000593750

http://hdl.handle.net/20.500.11750/16319
DOI
10.22677/thesis.200000593750
Degree
Master
Department
Brain and Cognitive Sciences
Publisher
DGIST
Related Researcher
  • 유성운 Yu, Seong-Woon
  • Research Interests Molecular mechanisms of neuronal cell death and neurodegeneration
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Department of Brain Sciences Theses Master

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