Title

The effects of Compound ADX and Compound ADY in neuroinflammation phenotypes of Presenilin 2 N141I knock-in mouse

DGIST Authors

Seohyeon Hwang ; Seong-Woon Yu ; Byung-Hoon Lee

Advisor

유성운

Co-Advisor(s)

Byung-Hoon Lee

Issued Date

2022

Awarded Date

2022/02

Citation

Seohyeon Hwang. (2022). The effects of Compound ADX and Compound ADY in neuroinflammation phenotypes of Presenilin 2 N141I knock-in mouse. doi: 10.22677/thesis.200000592247

Type

Thesis

Subject

Microglia, inflammation, Alzheimer disease (AD), Presenilin 2, Drug repositioning

Description

Microglia, inflammation, Alzheimer disease (AD), Presenilin 2, Drug repositioning

Table Of Contents

Ⅰ. Introduction 1
Ⅱ. Material and Methods 6
2.1 Mice 6
2.2 Cell culture 6
2.3 Reagents 7
2.4 Enzyme-linked immunosorbent assay (ELISA) 7
2.5 Cell death assay 7
2.6 Open field test 8
2.7 Y-maze assay 8
2.8 Aβ uptake and degradation 9
2.9 Phagocytosis Assay 9
2.10 Statistical analysis 10
Ⅲ. Results 11
3.1 Compound ADX and ADY reduce LPS-induced IL-6 production in Psen2 N141I/+ microglia 11
3.2 Compound ADX and ADY prevent LPS-induced memory deficit in Psen2 N141I/+ mice 14
3.3 Compound ADX and ADY rescue impaired degradation of fibril Aβ in Psen2 N141I/+ microglia 18
3.4 Compound ADX and ADY rescue the impaired phagocytosis ability in Psen2 N141I KI/+ microglia 22
Ⅳ. Discussion 26
Reference 28
Abstract in Korea 30

URI

http://dgist.dcollection.net/common/orgView/200000592247
http://hdl.handle.net/20.500.11750/16337

DOI

10.22677/thesis.200000592247

Degree

Master

Department

Brain and Cognitive Sciences

Publisher

DGIST

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