Attenuation of neuroinflammation by an antiarrhythmic drug through reduction of IL-6 in Presenilin 2 N141I knock-in Alzheimer disease model
- Title
- Attenuation of neuroinflammation by an antiarrhythmic drug through reduction of IL-6 in Presenilin 2 N141I knock-in Alzheimer disease model
- Alternative Title
- 가족성 알츠하이머 질병 마우스 모델에서 미세아교세포에 대한 compound D의 항염증효과
- Author(s)
- Youngjin Park
- DGIST Authors
- Youngjin Park ; Seong-Woon Yu ; Young-sam Lee
- Advisor
- 유성운
- Co-Advisor(s)
- Young-sam Lee
- Issued Date
- 2021
- Awarded Date
- 2021/02
- Type
- Thesis
- Table Of Contents
-
Ⅰ. Introduction 1
Ⅱ. Material and Methods 7
2.1 Animals 7
2.2 Cell culture 7
2.3 Reagents and antibodies 9
2.4 Cell death assay 9
2.5 Enzyme-linked immunosorbent assay 10
2.6 Quantitative RT-PCR 10
2.7 Western blot 12
2.8 Microglial transduction 12
2.9 Measurement of Aβ uptake and degradation 13
2.8 Statistical analysis 14
Ⅲ. Results 15
3.1 Compound D reduces neuroinflammation through recovery of REV-ERBα level in KI/+ microglia 15
3.2 Compound D regulates clock genes and clock-controlled cytokines in KI/+ microglia 21
3.3 Compound D reduces neuroinflammation in KI/+ macrophages 25
3.4 Compound D induces autophagy and Aβ clearance in microglia 29
3.5 Compound D recovers autophagy in LPS treated microglia 33
Ⅳ. Discussion 38
Ⅴ. Reference 43
Abstract in Korean 47
- URI
-
http://dgist.dcollection.net/common/orgView/200000361998
http://hdl.handle.net/20.500.11750/16652
- Degree
- Master
- Department
- Brain and Cognitive Sciences
- Publisher
- DGIST
- Related Researcher
-
-
Yu, Seong-Woon
- Research Interests Molecular mechanisms of neuronal cell death and neurodegeneration
-
- Files in This Item:
-
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- Appears in Collections:
- Department of Brain Sciences Theses Master
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