Title

Attenuation of neuroinflammation by an antiarrhythmic drug through reduction of IL-6 in Presenilin 2 N141I knock-in Alzheimer disease model

Alternative Title

가족성 알츠하이머 질병 마우스 모델에서 미세아교세포에 대한 compound D의 항염증효과

DGIST Authors

Youngjin Park ; Seong-Woon Yu ; Young-sam Lee

Advisor

유성운

Co-Advisor(s)

Young-sam Lee

Issued Date

2021

Awarded Date

2021/02

Citation

Youngjin Park. (2021). Attenuation of neuroinflammation by an antiarrhythmic drug through reduction of IL-6 in Presenilin 2 N141I knock-in Alzheimer disease model. doi: 10.22677/thesis.200000361998

Type

Thesis

Subject

Alzheimer’s disease (AD), microglia, neuroinflammation, autophagy, Anti-inflammatory or anti-AD drug screening, 알츠하이머병, 미세아교세포, 신경염증, 자가포식작용, 항 염증 또는 항 알츠하이머 약물 스크리닝

Table Of Contents

Ⅰ. Introduction 1
Ⅱ. Material and Methods 7
2.1 Animals 7
2.2 Cell culture 7
2.3 Reagents and antibodies 9
2.4 Cell death assay 9
2.5 Enzyme-linked immunosorbent assay 10
2.6 Quantitative RT-PCR 10
2.7 Western blot 12
2.8 Microglial transduction 12
2.9 Measurement of Aβ uptake and degradation 13
2.8 Statistical analysis 14
Ⅲ. Results 15
3.1 Compound D reduces neuroinflammation through recovery of REV-ERBα level in KI/+ microglia 15
3.2 Compound D regulates clock genes and clock-controlled cytokines in KI/+ microglia 21
3.3 Compound D reduces neuroinflammation in KI/+ macrophages 25
3.4 Compound D induces autophagy and Aβ clearance in microglia 29
3.5 Compound D recovers autophagy in LPS treated microglia 33
Ⅳ. Discussion 38
Ⅴ. Reference 43
Abstract in Korean 47

URI

http://dgist.dcollection.net/common/orgView/200000361998
http://hdl.handle.net/20.500.11750/16652

DOI

10.22677/thesis.200000361998

Degree

Master

Department

Brain and Cognitive Sciences

Publisher

DGIST

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