Title

Investigation of IQSEC3 functions in regulating GABAergic synapse development and depression-like behavior in mice

Alternative Title

억제성 시냅스 발달과 공간 기억력 조절하는 IQSEC3의 역할 규명

DGIST Authors

Hyeji Jung ; Ji Won Um ; Jinsoo Seo

Advisor

엄지원

Co-Advisor(s)

Jinsoo Seo

Issued Date

2021

Awarded Date

2021/02

Citation

Hyeji Jung. (2021). Investigation of IQSEC3 functions in regulating GABAergic synapse development and depression-like behavior in mice. doi: 10.22677/thesis.200000364357

Type

Thesis

Subject

IQSEC3, spatial memory, Depression, GABAergic synapse, IQSEC3, 공간 기억, 우울증, GABA 성 시냅스

Table Of Contents

I. Introduction 1
1.1 E/I balance in neural network 1
1.2 Inhibitory synaptic structure and organizers 2
1.3 Functional role of IQSEC3 3

Ⅱ. Materials and methods 5
2.1 Animals 5
2.2 Stereotactic virus injection 5
2.3 Immunohistochemistry 6
2.4 Seizure test 6
2.5 LABORAS home cage activity analysis 6
2.6 Behavioral tests 7
2.7 Drug administration 9

III. Results 10
3.1 Generation of Iqsec3 conditional knockout (cKO) mice 10
3.2 Decreased GABAergic synapses in hippocampal CA1 of Nestin-Iqsec3 mice 10
3.3 Selective reduction in symmetric synaptic structures in hippocampal CA1 of Nestin-Iqsec3 mice 11
3.4 Increased seizure susceptibility of Nestin-Iqsec3 mice 11
3.5 Impaired spatial memory of Nestin-Iqsec3 mice 12
3.6 No alterations in home cage activities of Nestin-Iqsec3 mice 12
3.7 No alterations in anxiety-related behaviors of Nestin-Iqsec3 mice 12
3.8 No alterations in memories and social behaviors of Nestin-Iqsec3 mice 12
3.9 Decreased depressive-like behaviors of Nestin-Iqsec3 mice 13
3.10 Antidepressive-like behaviors of Nestin-Iqsec3 mice responded to valproic acid but not to rapamycin 13
3.11 No alteration in depressive-like behaviors of mice with Iqsec3 deletion in SST-IN 14
3.12 Reduction in GABAergic synapses in mPFC of Nestin-Iqsec3 mice 14
3.13 mPFC-specific Iqsec3 KO mice recapitulated antidepressive-like behavior 15

Ⅳ. Discussion 30

V. Reference 32

Ⅵ. 요 약 문 35

URI

http://dgist.dcollection.net/common/orgView/200000364357
http://hdl.handle.net/20.500.11750/16692

DOI

10.22677/thesis.200000364357

Degree

Master

Department

Brain and Cognitive Sciences

Publisher

DGIST

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