Investigation of IQSEC3 functions in regulating GABAergic synapse development and depression-like behavior in mice
- Title
- Investigation of IQSEC3 functions in regulating GABAergic synapse development and depression-like behavior in mice
- Alternative Title
- 억제성 시냅스 발달과 공간 기억력 조절하는 IQSEC3의 역할 규명
- Author(s)
- Hyeji Jung
- DGIST Authors
- Hyeji Jung ; Ji Won Um ; Jinsoo Seo
- Advisor
- 엄지원
- Co-Advisor(s)
- Jinsoo Seo
- Issued Date
- 2021
- Awarded Date
- 2021/02
- Type
- Thesis
- Table Of Contents
-
I. Introduction 1
1.1 E/I balance in neural network 1
1.2 Inhibitory synaptic structure and organizers 2
1.3 Functional role of IQSEC3 3
Ⅱ. Materials and methods 5
2.1 Animals 5
2.2 Stereotactic virus injection 5
2.3 Immunohistochemistry 6
2.4 Seizure test 6
2.5 LABORAS home cage activity analysis 6
2.6 Behavioral tests 7
2.7 Drug administration 9
III. Results 10
3.1 Generation of Iqsec3 conditional knockout (cKO) mice 10
3.2 Decreased GABAergic synapses in hippocampal CA1 of Nestin-Iqsec3 mice 10
3.3 Selective reduction in symmetric synaptic structures in hippocampal CA1 of Nestin-Iqsec3 mice 11
3.4 Increased seizure susceptibility of Nestin-Iqsec3 mice 11
3.5 Impaired spatial memory of Nestin-Iqsec3 mice 12
3.6 No alterations in home cage activities of Nestin-Iqsec3 mice 12
3.7 No alterations in anxiety-related behaviors of Nestin-Iqsec3 mice 12
3.8 No alterations in memories and social behaviors of Nestin-Iqsec3 mice 12
3.9 Decreased depressive-like behaviors of Nestin-Iqsec3 mice 13
3.10 Antidepressive-like behaviors of Nestin-Iqsec3 mice responded to valproic acid but not to rapamycin 13
3.11 No alteration in depressive-like behaviors of mice with Iqsec3 deletion in SST-IN 14
3.12 Reduction in GABAergic synapses in mPFC of Nestin-Iqsec3 mice 14
3.13 mPFC-specific Iqsec3 KO mice recapitulated antidepressive-like behavior 15
Ⅳ. Discussion 30
V. Reference 32
Ⅵ. 요 약 문 35
- URI
-
http://dgist.dcollection.net/common/orgView/200000364357
http://hdl.handle.net/20.500.11750/16692
- Degree
- Master
- Department
- Brain and Cognitive Sciences
- Publisher
- DGIST
- Related Researcher
-
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Um, Ji Won
- Research Interests Molecular and cellular mechanisms underlying synapse elimination; Key synaptic mechanisms associated with Alzheimer's disease and autism spectrum disorders; Synaptic homeostasis
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- Appears in Collections:
- Department of Brain Sciences Theses Master
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