Hyeji Jung. (2021). Investigation of IQSEC3 functions in regulating GABAergic synapse development and depression-like behavior in mice. doi: 10.22677/thesis.200000364357
I. Introduction 1 1.1 E/I balance in neural network 1 1.2 Inhibitory synaptic structure and organizers 2 1.3 Functional role of IQSEC3 3
Ⅱ. Materials and methods 5 2.1 Animals 5 2.2 Stereotactic virus injection 5 2.3 Immunohistochemistry 6 2.4 Seizure test 6 2.5 LABORAS home cage activity analysis 6 2.6 Behavioral tests 7 2.7 Drug administration 9
III. Results 10 3.1 Generation of Iqsec3 conditional knockout (cKO) mice 10 3.2 Decreased GABAergic synapses in hippocampal CA1 of Nestin-Iqsec3 mice 10 3.3 Selective reduction in symmetric synaptic structures in hippocampal CA1 of Nestin-Iqsec3 mice 11 3.4 Increased seizure susceptibility of Nestin-Iqsec3 mice 11 3.5 Impaired spatial memory of Nestin-Iqsec3 mice 12 3.6 No alterations in home cage activities of Nestin-Iqsec3 mice 12 3.7 No alterations in anxiety-related behaviors of Nestin-Iqsec3 mice 12 3.8 No alterations in memories and social behaviors of Nestin-Iqsec3 mice 12 3.9 Decreased depressive-like behaviors of Nestin-Iqsec3 mice 13 3.10 Antidepressive-like behaviors of Nestin-Iqsec3 mice responded to valproic acid but not to rapamycin 13 3.11 No alteration in depressive-like behaviors of mice with Iqsec3 deletion in SST-IN 14 3.12 Reduction in GABAergic synapses in mPFC of Nestin-Iqsec3 mice 14 3.13 mPFC-specific Iqsec3 KO mice recapitulated antidepressive-like behavior 15
