The role of acetylcholine in the mPFC and the PPC during working memory: The behavioral analysis of pharmacological and optogenetical manipulation of neural activity and cholinergic modulation
Title
The role of acetylcholine in the mPFC and the PPC during working memory: The behavioral analysis of pharmacological and optogenetical manipulation of neural activity and cholinergic modulation
Alternative Title
단기 기억에서 아세틸콜린의 역할: 약물 또는 광유전학을 이용한 활성 조절과 아세틸콜린 조절을 통한 행동학적 분석
The posterior parietal cortex (PPC) together with the prefrontal cortex (PFC) has been known as essential regions for cognitive functions such as attention, navigation, decision-making, as well as working memory (WM). Lesion of the PPC or the PFC leads to a significant WM im-pairment in various animals. Particularly, the critical function of the cholinergic innervation in the PFC has been demonstrated in chemically or genetically depleted animal models, yet the func-tion of acetylcholine in PPC has never been explored thoroughly.
Table Of Contents
Ⅰ. Introduction 1 1.1 Working memory 1 1.2 Acetylcholine in WM 1 1.3 Psychophysical experiments 2 Ⅱ. Materials and Method 3 2.1 Animals 3 2.2 Stereotaxic surgery 3 2.3 Two-alternative forced-choice task 3 2.4 Behavioral experiments 4 2.4.1 Pharmacological inactivation 4 2.4.2 Optogenetic inactivation 4 2.5 Ex vivo whole-cell recording 5 2.5.1 Sustained activity with drug application 5 2.5.2 Photoinhibition of the pyramidal neurons in the mPFC 2/3 layer 5 2.6 Data analysis and statistics 6 Ⅲ. Results 7 Ⅳ. Discussion 28 References 31 요약문 35