The role of acetylcholine in the mPFC and the PPC during working memory: The behavioral analysis of pharmacological and optogenetical manipulation of neural activity and cholinergic modulation
The role of acetylcholine in the mPFC and the PPC during working memory: The behavioral analysis of pharmacological and optogenetical manipulation of neural activity and cholinergic modulation
Alternative Title
단기 기억에서 아세틸콜린의 역할: 약물 또는 광유전학을 이용한 활성 조절과 아세틸콜린 조절을 통한 행동학적 분석
Yeri Kim. (2021). The role of acetylcholine in the mPFC and the PPC during working memory: The behavioral analysis of pharmacological and optogenetical manipulation of neural activity and cholinergic modulation. doi: 10.22677/thesis.200000364571
The posterior parietal cortex (PPC) together with the prefrontal cortex (PFC) has been known as essential regions for cognitive functions such as attention, navigation, decision-making, as well as working memory (WM). Lesion of the PPC or the PFC leads to a significant WM im-pairment in various animals. Particularly, the critical function of the cholinergic innervation in the PFC has been demonstrated in chemically or genetically depleted animal models, yet the func-tion of acetylcholine in PPC has never been explored thoroughly.
Table Of Contents
Ⅰ. Introduction 1 1.1 Working memory 1 1.2 Acetylcholine in WM 1 1.3 Psychophysical experiments 2 Ⅱ. Materials and Method 3 2.1 Animals 3 2.2 Stereotaxic surgery 3 2.3 Two-alternative forced-choice task 3 2.4 Behavioral experiments 4 2.4.1 Pharmacological inactivation 4 2.4.2 Optogenetic inactivation 4 2.5 Ex vivo whole-cell recording 5 2.5.1 Sustained activity with drug application 5 2.5.2 Photoinhibition of the pyramidal neurons in the mPFC 2/3 layer 5 2.6 Data analysis and statistics 6 Ⅲ. Results 7 Ⅳ. Discussion 28 References 31 요약문 35
