DGIST Scholar: The role of acetylcholine in the mPFC and the PPC during working memory: The behavioral analysis of pharmacological and optogenetical manipulation of neural activity and cholinergic modulation

Department of Brain Sciences Theses Master

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The role of acetylcholine in the mPFC and the PPC during working memory: The behavioral analysis of pharmacological and optogenetical manipulation of neural activity and cholinergic modulation

Title: The role of acetylcholine in the mPFC and the PPC during working memory: The behavioral analysis of pharmacological and optogenetical manipulation of neural activity and cholinergic modulation

Alternative Title: 단기 기억에서 아세틸콜린의 역할: 약물 또는 광유전학을 이용한 활성 조절과 아세틸콜린 조절을 통한 행동학적 분석

Author(s): Yeri Kim

DGIST Authors: Yeri Kim ; Cheil Moon ; Jong-Cheol Rah

Advisor: 문제일

Co-Advisor(s): Jong-Cheol Rah

Issued Date: 2021

Awarded Date: 2021/02

Type: Thesis

Subject: Working-Memory, Medial prefrontal cortex, Posterior cingulate cortex, Acetylcholine, 전전두피질, 후두정피질, 아세틸콜린, 패치클램프, 광유전학

Abstract: The posterior parietal cortex (PPC) together with the prefrontal cortex (PFC) has been known as essential regions for cognitive functions such as attention, navigation, decision-making, as well as working memory (WM). Lesion of the PPC or the PFC leads to a significant WM im-pairment in various animals. Particularly, the critical function of the cholinergic innervation in the PFC has been demonstrated in chemically or genetically depleted animal models, yet the func-tion of acetylcholine in PPC has never been explored thoroughly.

Table Of Contents: Ⅰ. Introduction 1
1.1 Working memory 1
1.2 Acetylcholine in WM 1
1.3 Psychophysical experiments 2
Ⅱ. Materials and Method 3
2.1 Animals 3
2.2 Stereotaxic surgery 3
2.3 Two-alternative forced-choice task 3
2.4 Behavioral experiments 4
2.4.1 Pharmacological inactivation 4
2.4.2 Optogenetic inactivation 4
2.5 Ex vivo whole-cell recording 5
2.5.1 Sustained activity with drug application 5
2.5.2 Photoinhibition of the pyramidal neurons in the mPFC 2/3 layer 5
2.6 Data analysis and statistics 6
Ⅲ. Results 7
Ⅳ. Discussion 28
References 31
요약문 35