Title

Structural characterization of mouse BEST1 channel

DGIST Authors

Ara Ko ; Kyuhyung Kim ; Hyun-Ho Lim

Advisor

김규형

Co-Advisor(s)

Hyun-Ho Lim

Issued Date

2022

Awarded Date

2022/08

Citation

Ara Ko. (2022). Structural characterization of mouse BEST1 channel. doi: 10.22677/thesis.200000629353

Type

Thesis

Subject

Bestrophin-1, Calcium-activated chloride channel, Cryo-EM

Description

Bestrophin-1, Calcium-activated chloride channel, Cryo-EM

Abstract

더보기

Table Of Contents

Ⅰ. INTRODUCTION 1
Ⅱ. MATERIALS AND METHODS 5
2.1 Plasmid construct 5
2.2 Cell culture and transfection 5
2.3 Western Blot analysis 6
2.4 Protein expression and purification in mammalian cell 6
2.5 Negative stain TEM 7
2.6 Cryo-EM data collection and image processing 8
2.7 Protein expression and purification in bacterial cells 9
Ⅲ. RESULTS 14
3.1 Detergent screening and protein purification of mBEST1 14
3.2 Cryo-EM structures of mBEST1 17
3.3 Neck and Ca2+-clasp of mBEST1 and cBEST1 21
3.4 The aperture region of mBEST1 25
3.5 Cl- binding in mBEST1 26
3.6 The C-terminus of mBEST1 27
Ⅳ. DISCUSSION 32
Ⅴ. REFERENCES 35
Ⅵ. Abstract in Korea (요약문) 39

URI

http://dgist.dcollection.net/common/orgView/200000629353
http://hdl.handle.net/20.500.11750/16791

DOI

10.22677/thesis.200000629353

Degree

Master

Department

Department of Brain Sciences

Publisher

DGIST

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