Structural characterization of mouse BEST1 channel
- Title
- Structural characterization of mouse BEST1 channel
- Author(s)
- Ara Ko
- DGIST Authors
- Ara Ko ; Kyuhyung Kim ; Hyun-Ho Lim
- Advisor
- 김규형
- Co-Advisor(s)
- Hyun-Ho Lim
- Issued Date
- 2022
- Awarded Date
- 2022/08
- Type
- Thesis
- Description
- Bestrophin-1, Calcium-activated chloride channel, Cryo-EM
- Abstract
- Table Of Contents
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Ⅰ. INTRODUCTION 1
Ⅱ. MATERIALS AND METHODS 5
2.1 Plasmid construct 5
2.2 Cell culture and transfection 5
2.3 Western Blot analysis 6
2.4 Protein expression and purification in mammalian cell 6
2.5 Negative stain TEM 7
2.6 Cryo-EM data collection and image processing 8
2.7 Protein expression and purification in bacterial cells 9
Ⅲ. RESULTS 14
3.1 Detergent screening and protein purification of mBEST1 14
3.2 Cryo-EM structures of mBEST1 17
3.3 Neck and Ca2+-clasp of mBEST1 and cBEST1 21
3.4 The aperture region of mBEST1 25
3.5 Cl- binding in mBEST1 26
3.6 The C-terminus of mBEST1 27
Ⅳ. DISCUSSION 32
Ⅴ. REFERENCES 35
Ⅵ. Abstract in Korea (요약문) 39
- URI
-
http://dgist.dcollection.net/common/orgView/200000629353
http://hdl.handle.net/20.500.11750/16791
- Degree
- Master
- Department
- Department of Brain Sciences
- Publisher
- DGIST
- Related Researcher
-
-
Kim, Kyuhyung
- Research Interests Neurobehavior; Neural Circuit; Neurodevelopment; 신경회로; 신경행동; 신경발생; 신경유전학
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- Files in This Item:
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- Appears in Collections:
- Department of Brain Sciences Theses Master
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