Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Kim, Haemin | - |
dc.contributor.author | Youn, Sangyeon | - |
dc.contributor.author | Kim, Jinwoo | - |
dc.contributor.author | Park, Sunghun | - |
dc.contributor.author | Lee, Moonhwan | - |
dc.contributor.author | Hwang, Jae Youn | - |
dc.contributor.author | Chang, Jin Ho | - |
dc.date.accessioned | 2023-01-03T19:40:14Z | - |
dc.date.available | 2023-01-03T19:40:14Z | - |
dc.date.created | 2022-09-23 | - |
dc.date.issued | 2022-11 | - |
dc.identifier.issn | 1749-4885 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/17286 | - |
dc.description.abstract | Although laser scanning microscopy is a pivotal imaging tool in biomedical research, optical scattering from tissue limits the depth of the imaging. To overcome this limitation, we propose a scheme called ultrasound-induced optical clearing microscopy, which makes use of temporary, localized optical clearing based on ultrasound-induced gas bubbles. In this method, bubbles are generated by high-intensity pulsed ultrasound at a desired depth and subsequently maintained by low-intensity continuous ultrasound during imaging. As a result, optical scattering and unwanted changes in the propagation direction of the incident photons are minimized in the bubble cloud, and thus the laser can be tightly focused at a deeper imaging plane. Through phantom and ex vivo experiments, we demonstrate that ultrasound-induced optical clearing microscopy is capable of increasing the imaging depth by a factor of six or more, while the resolution is similar to that of conventional laser scanning microscopy. Optical clearing based on ultrasound-induced gas bubbles offers new opportunities for deeper laser scanning microscopy of biological tissue. © 2022, The Author(s), under exclusive licence to Springer Nature Limited. | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.title | Deep laser microscopy using optical clearing by ultrasound-induced gas bubbles | - |
dc.type | Article | - |
dc.identifier.doi | 10.1038/s41566-022-01068-x | - |
dc.identifier.scopusid | 2-s2.0-85137472598 | - |
dc.identifier.bibliographicCitation | Nature Photonics, v.16, no.11, pp.762 - 768 | - |
dc.description.isOpenAccess | FALSE | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | TISSUE | - |
dc.subject.keywordPlus | CAVITATION | - |
dc.subject.keywordPlus | HISTOTRIPSY | - |
dc.citation.endPage | 768 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 762 | - |
dc.citation.title | Nature Photonics | - |
dc.citation.volume | 16 | - |
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